Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (ab248090)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7091] to HMGN2 - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-HMGN2 antibody [EPR7091] - BSA and Azide free
    See all HMGN2 primary antibodies

  • Description

    Rabbit monoclonal [EPR7091] to HMGN2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248090 is the carrier-free version of ab124997 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248090 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR7091

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (ab248090)
    Western blot - Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (ab248090)
    All lanes : Anti-HMGN2 antibody [EPR7091] (ab124997) at 1/1000 dilution (Purified)

    Lane 1 : HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate prepared in 1%SDS Hot lysis method
    Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate prepared in 1%SDS Hot lysis method

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 9 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab124997, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (ab248090)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (ab248090)
    This data was developed using ab124997, the same antibody clone in a different buffer formulation.ab124997 at 1/250 dilution staining HMGN2 in paraffin-embedded Human colon tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Western blot - Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (ab248090)
    Western blot - Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (ab248090)
    All lanes : Anti-HMGN2 antibody [EPR7091] (ab124997) at 1/1000 dilution

    Lane 1 : HL60 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : SH-SY5Y cell lysate
    Lane 5 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 9 kDa



    This data was developed using ab124997, the same antibody clone in a different buffer formulation.

    All cell lysates were prepared in 1% SDS Hot lysis method.

  • Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (ab248090)
    Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (ab248090)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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