Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (ab248090)
![Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (ab248090)](https://www.abcam.com/ps/products/248/ab248090/Images/ab248090-406309-anti-hmgn2-antibody-epr7091-bsa-and-azide-free-western-blot-human.jpg "Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (ab248090)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7091] to HMGN2 - BSA and Azide free
- Suitable for: ICC, IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-HMGN2 antibody [EPR7091] - BSA and Azide free
See all HMGN2 primary antibodies -
Description
Rabbit monoclonal [EPR7091] to HMGN2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248090 is the carrier-free version of ab124997 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab248090 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR7091 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (ab248090)All lanes : Anti-HMGN2 antibody [EPR7091] (ab124997) at 1/1000 dilution (Purified)
Lane 1 : HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate prepared in 1%SDS Hot lysis method
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate prepared in 1%SDS Hot lysis method
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 9 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?This data was developed using ab124997, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (ab248090)](https://www.abcam.com/ps/products/248/ab248090/Images/ab248090-2-anti-hmgn2-antibody-epr7091-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (ab248090) -
![Western blot - Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (ab248090)](https://www.abcam.com/ps/products/248/ab248090/Images/ab248090-399119-anti-hmgn2-antibody-epr7091-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (ab248090)All lanes : Anti-HMGN2 antibody [EPR7091] (ab124997) at 1/1000 dilution
Lane 1 : HL60 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : SH-SY5Y cell lysate
Lane 5 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 9 kDaThis data was developed using ab124997, the same antibody clone in a different buffer formulation.
All cell lysates were prepared in 1% SDS Hot lysis method.
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![Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (ab248090)](https://www.abcam.com/ps/products/248/ab248090/Images/ab248090-4-benefits-of-recombinant-antibodies.png)
Anti-HMGN2 antibody [EPR7091] - BSA and Azide free (ab248090)
