Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-28 + IL-29 antibody [EPR14608] (ab191426)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14608] to IL-28 + IL-29
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Recombinant fragment

Overview

  • Product name

    Anti-IL-28 + IL-29 antibody [EPR14608]
    See all IL-28 + IL-29 primary antibodies

  • Description

    Rabbit monoclonal [EPR14608] to IL-28 + IL-29

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    WB
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IL28 recombinant protein; RAW 264.7 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-IL-28 + IL-29 antibody [EPR14608] (ab191426)
    Western blot - Anti-IL-28 + IL-29 antibody [EPR14608] (ab191426)
    Anti-IL-28 + IL-29 antibody [EPR14608] (ab191426) at 1/50000 dilution + IL28A recombinant protein at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 22 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-IL-28 + IL-29 antibody [EPR14608] (ab191426)
    Immunocytochemistry/ Immunofluorescence - Anti-IL-28 + IL-29 antibody [EPR14608] (ab191426)

    Immunofluorescent analysis of RAW 264.7cells (4% Paraformaldehyde-fixed; 0.1% tritonX-100 permeabilized) labeling IL28 + IL29 with ab191426 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution (ab150077) and counter-stained with DAPI (blue).

    Negative controls: anti-IL-28A at 1/100 dilution, followed by Goat anti mouse IgG Alexa Fluor®594 secondary at 1/400 dilution.

  • Flow Cytometry - Anti-IL-28 + IL-29 antibody [EPR14608] (ab191426)
    Flow Cytometry - Anti-IL-28 + IL-29 antibody [EPR14608] (ab191426)

    Flow Cytometry analysis of Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling IL28 + IL29 with purified ab191426 at 1/200 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised using 90% methanol. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).

  • Anti-IL-28 + IL-29 antibody [EPR14608] (ab191426)
    Anti-IL-28 + IL-29 antibody [EPR14608] (ab191426)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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