Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H1.0 antibody [EPR6536] - BSA and Azide free (ab203946)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6536] to Histone H1.0 - BSA and Azide free
  • Suitable for: ICC/IF, WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Histone H1.0 antibody [EPR6536] - BSA and Azide free
    See all Histone H1.0 primary antibodies

  • Description

    Rabbit monoclonal [EPR6536] to Histone H1.0 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431 and HeLa cell lysates; Human fetal kidney and BxPC3 cell lysates. IHC-P: Human colon tissue. ICC/IF: BxPC3 cells.

  • General notes

    Ab203946 is the carrier-free version of ab134914. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab203946 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Histone H1.0 antibody [EPR6536] - BSA and Azide free (ab203946)
    Western blot - Anti-Histone H1.0 antibody [EPR6536] - BSA and Azide free (ab203946)
    All lanes : Anti-Histone H1.0 antibody [EPR6536] (ab134914) at 1/1000 dilution

    Lane 1 : Wild-type A431 cell lysate
    Lane 2 : H1F0 knockout A431 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 21 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab134914).

    Lanes 1 - 3: Merged signal (red and green). Green - ab134914 observed at 30 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

    ab134914 was shown to react with Histone H1.0 in wild-type A431 cells in Western blot. Loss of signal was observed when H1F0 knockout sample was used. Wild-type and H1F0 knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab134914 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Anti-Histone H1.0 antibody [EPR6536] - BSA and Azide free (ab203946)
    Anti-Histone H1.0 antibody [EPR6536] - BSA and Azide free (ab203946)

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