Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

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Anti-gamma H2A.X (phospho S139) antibody [BLR053F] (ab243906)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR053F] to gamma H2A.X (phospho S139)
Suitable for: WB, ICC, IHC-P, IP
Reacts with: Mouse, Human

Product name

Anti-gamma H2A.X (phospho S139) antibody [BLR053F]
See all gamma H2A.X primary antibodies
Description

Rabbit monoclonal [BLR053F] to gamma H2A.X (phospho S139)
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Mouse Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human gamma H2A.X. Surrounding Serine 139. NP_002096.1 and Gene ID 3014.
Database link: P16104
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR053F
Isotype

IgG
Research areas

Immunoprecipitation - Anti-gamma H2A.X (phospho S139) antibody [BLR053F] (ab243906)

Gamma H2A.Z was immunoprecipitated from 1.0 mg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab243906 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243864 at 1/1000 dilution.

Lane 1: ab243906 IP in Jurkat (untreated) whole cell lysate.

Lane 2: ab243906 IP in Jurkat (treated with Etoposide) whole cell lysate.

Lane 3: Control IgG IP in Jurkat whole cell lysate.

Detection: Chemiluminescence with an exposure time of 3 seconds.
Western blot - Anti-gamma H2A.X (phospho S139) antibody [BLR053F] (ab243906)

Western blot analysis using ab243906 at 1/1000 dilution.

Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) (untreated) whole cell lysate (50 µg) .

Lane 2: Jurkat (Etoposide-treated) whole cell lysate (50 µg).

A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary.
Immunocytochemistry - Anti-gamma H2A.X (phospho S139) antibody [BLR053F] (ab243906)

Formalin-fixed, paraffin-embedded HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling gamma H2A.X (phospho S139) using ab243906 at 1/100 dilution in ICC analysis. An HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.

Left panel: Untreated HeLa cells

Right panel: Etoposide treated HeLa cells
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody [BLR053F] (ab243906)

Formalin-fixed, paraffin-embedded mouse CT26 colon carcinoma tissue stained for H2A.X (phospho S139) using ab243906 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody [BLR053F] (ab243906)

Formalin-fixed, paraffin-embedded human G203basal cell carcinoma tissue stained for H2A.X (phospho S139) using ab243906 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Anti-gamma H2A.X (phospho S139) antibody [BLR053F] (ab243906)

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