Anti-gamma H2A.X (phospho S139) antibody [BLR053F] (ab243906)
![Anti-gamma H2A.X (phospho S139) antibody [BLR053F] (ab243906)](https://www.abcam.com/ps/products/243/ab243906/Images/ab243906-334415-anti-gamma-h2ax-antibody-blr053f-immunoprecipitation.jpg "Anti-gamma H2A.X (phospho S139) antibody [BLR053F] (ab243906)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR053F] to gamma H2A.X (phospho S139)
- Suitable for: WB, ICC, IHC-P, IP
- Reacts with: Mouse, Human
Overview
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Product name
Anti-gamma H2A.X (phospho S139) antibody [BLR053F]
See all gamma H2A.X primary antibodies -
Description
Rabbit monoclonal [BLR053F] to gamma H2A.X (phospho S139) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P MouseHumanIP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human gamma H2A.X. Surrounding Serine 139. NP_002096.1 and Gene ID 3014.
Database link: P16104 -
General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR053F -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-gamma H2A.X (phospho S139) antibody [BLR053F] (ab243906)
Gamma H2A.Z was immunoprecipitated from 1.0 mg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab243906 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243864 at 1/1000 dilution.
Lane 1: ab243906 IP in Jurkat (untreated) whole cell lysate.
Lane 2: ab243906 IP in Jurkat (treated with Etoposide) whole cell lysate.
Lane 3: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 seconds.
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![Western blot - Anti-gamma H2A.X (phospho S139) antibody [BLR053F] (ab243906)](https://www.abcam.com/ps/products/243/ab243906/Images/ab243906-334414-anti-gamma-h2ax-antibody-blr053f-western-blot.jpg)
Western blot - Anti-gamma H2A.X (phospho S139) antibody [BLR053F] (ab243906)Western blot analysis using ab243906 at 1/1000 dilution.
Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) (untreated) whole cell lysate (50 µg) .
Lane 2: Jurkat (Etoposide-treated) whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary.
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![Immunocytochemistry - Anti-gamma H2A.X (phospho S139) antibody [BLR053F] (ab243906)](https://www.abcam.com/ps/products/243/ab243906/Images/ab243906-334413-anti-gamma-h2ax-antibody-blr053f-immunocytochemistry.jpg)
Immunocytochemistry - Anti-gamma H2A.X (phospho S139) antibody [BLR053F] (ab243906)Formalin-fixed, paraffin-embedded HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling gamma H2A.X (phospho S139) using ab243906 at 1/100 dilution in ICC analysis. An HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
Left panel: Untreated HeLa cells
Right panel: Etoposide treated HeLa cells
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody [BLR053F] (ab243906)](https://www.abcam.com/ps/products/243/ab243906/Images/ab243906-334412-anti-gamma-h2ax-antibody-blr053f-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody [BLR053F] (ab243906)Formalin-fixed, paraffin-embedded mouse CT26 colon carcinoma tissue stained for H2A.X (phospho S139) using ab243906 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody [BLR053F] (ab243906)](https://www.abcam.com/ps/products/243/ab243906/Images/ab243906-334411-anti-gamma-h2ax-antibody-blr053f-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody [BLR053F] (ab243906)Formalin-fixed, paraffin-embedded human G203basal cell carcinoma tissue stained for H2A.X (phospho S139) using ab243906 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
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![Anti-gamma H2A.X (phospho S139) antibody [BLR053F] (ab243906)](https://www.abcam.com/ps/products/243/ab243906/Images/ab243906-6-benefits-of-recombinant-antibodies.png)
Anti-gamma H2A.X (phospho S139) antibody [BLR053F] (ab243906)
