Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IP10 antibody [EPR23493-29] - BSA and Azide free (ab273514)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23493-29] to IP10 - BSA and Azide free
  • Suitable for: Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-IP10 antibody [EPR23493-29] - BSA and Azide free
    See all IP10 primary antibodies

  • Description

    Rabbit monoclonal [EPR23493-29] to IP10 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IPmore details
    Unsuitable for: ICC/IF,IHC-P or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: Human peripheral blood mononuclear cell (PBMC) treated with 10ng/ml IFN-gamma for 1h, then add 300ng/ml Brefeldin A (BFA) for another 3h and THP-1 treated with 200ng/ml IFN gamma, 50ng/ml lipopolysaccharide (LPS) and protein transport inhibitor cocktail for 24h. IP: THP-1 (human monocytic leukemia monocyte) treated with 200ng/ml IFN-r and 50ng/ml LPS for 24 hours, culture supernatant.

  • General notes

    ab273514 is the carrier-free version of ab254374. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-IP10 antibody [EPR23493-29] - BSA and Azide free (ab273514)
    Flow Cytometry - Anti-IP10 antibody [EPR23493-29] - BSA and Azide free (ab273514)

    Flow cytometric analysis of 2% paraformaldehyde fixed, 0.1% Tween-20 permeabilized Human peripheral blood mononuclear (PBMC) (treated with 10ng/ml IFN-gamma for 1h, then add 300ng/ml Brefeldin A (BFA) for another 3h) cells labelling IP10 with ab254374 at 1/50 dilution (1 ug) (Right) compared with a Rabbit monoclonal IgG  (ab172730) isotype control (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with anti-CD14 conjugated to BV510. Then fixed with 2% PFA followed by intracellularly stained with rabbit IgG (Left) or ab254374 (Right).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254374).

  • Immunoprecipitation - Anti-IP10 antibody [EPR23493-29] - BSA and Azide free (ab273514)
    Immunoprecipitation - Anti-IP10 antibody [EPR23493-29] - BSA and Azide free (ab273514)

    IP10 was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) (treated with 200ng/ml IFN-r and 50ng/ml LPS for 24 hours) supernatant with ab254374 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254374 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: THP-1 treated with 200ng/ml IFN-r and 50ng/ml LPS for 24 hours, supernatant 10 ug

    Lane 2: 254374 IP in THP-1 treated with 200ng/ml IFN-r and 50ng/ml LPS for 24 hours, supernatant

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254374 in THP-1 treated with 200ng/ml IFN-r and 50ng/ml LPS for 24 hours, supernatant

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254374).

  • Flow Cytometry - Anti-IP10 antibody [EPR23493-29] - BSA and Azide free (ab273514)
    Flow Cytometry - Anti-IP10 antibody [EPR23493-29] - BSA and Azide free (ab273514)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized THP-1 (Human monocytic leukemia monocyte) (treated with 200ng/ml IFN gamma, 50ng/ml lipopolysaccharide (LPS) and protein transport inhibitor Cocktail for 24h) (Right) / Untreated control (Left)  labelling IP10 with ab254374 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254374).

  • Anti-IP10 antibody [EPR23493-29] - BSA and Azide free (ab273514)
    Anti-IP10 antibody [EPR23493-29] - BSA and Azide free (ab273514)

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