Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)
![Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)](https://www.abcam.com/ps/products/275/ab275960/Images/ab275960-411925-antitbr2-eomes-antibody-blr104h-immunoprecipitation-human.jpg "Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR104H] to TBR2 / Eomes - BSA free
- Suitable for: WB, IP, IHC-P, ICC
- Reacts with: Human
Overview
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Product name
Anti-TBR2 / Eomes antibody [BLR104H] - BSA free
See all TBR2 / Eomes primary antibodies -
Description
Rabbit monoclonal [BLR104H] to TBR2 / Eomes - BSA free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P HumanIP HumanWB Human
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Immunogen
Synthetic peptide within Human TBR2/ Eomes aa 125-175. The exact sequence is proprietary. Genbank: NP_001265111.1
Database link: O95936 -
Positive control
- WB: NK-92 whole cell lysate. IP: NK-92 whole cell lysate. IHC-P: Human tonsil and non-Hodgkin's B-cell lymphoma tissue. ICC: NK-92 cells
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General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.80
Preservative: 0.09% Sodium azide
Constituent: 99.91% Borate buffered saline -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR104H -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)
Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from NK-92 cells was
prepared using NETN lysis buffer. ab275960 used for IP at 6 µl per reaction. For blotting immunoprecipitated TBR2 / Eomes, ab275960 was used at 1/1000 dilution.Lane 1: ab275960 IP
Lane 2: Control IgG IP
Chemiluminescence with an exposure time of 10 seconds.
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![Western blot - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)](https://www.abcam.com/ps/products/275/ab275960/Images/ab275960-411924-antitbr2-eomes-antibody-blr104h-western-blot-human.jpg)
Western blot - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)All lanes : Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 3 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate at 50 µg
Lane 4 : NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) whole cell lysate at 10 µg
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Predicted band size: 72 kDa
Exposure time: 75 seconds
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![Immunocytochemistry - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)](https://www.abcam.com/ps/products/275/ab275960/Images/ab275960-411928-AntiTBR2-Eomes-antibody-BLR104H--BSA-free-immunocytochemistry-NK92-cells.JPG)
Immunocytochemistry - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) cells stained for TBR2 / Eomes using ab275960 at 1/100 dilution in immunocytochemistry. Secondary antibody is a HRP-conjugated Goat anti-Rabbit IgG. DAB substrate.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)](https://www.abcam.com/ps/products/275/ab275960/Images/ab275960-411927-AntiTBR2-Eomes-antibody-BLR104H--BSA-free-immunohistochemistry-non-hodgkins-B-cell-lymphoma.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)Formalin-fixed, paraffin-embedded non-Hodgkin's B-cell lymphoma tissue stained for TBR2 / Eomes using ab275960 at 1/100 dilution in immunohistochemical analysis. Secondary antibody is a HRP-conjugated Goat anti-Rabbit IgG. DAB substrate.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)](https://www.abcam.com/ps/products/275/ab275960/Images/ab275960-411926-AntiTBR2-Eomes-antibody-BLR104H--BSA-free-immunohistochemistry-tonsil.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)Formalin-fixed, paraffin-embedded human tonsil tissue stained for TBR2 / Eomes using ab275960 at 1/100 dilution in immunohistochemical analysis. Secondary antibody is a HRP-conjugated Goat anti-Rabbit IgG. DAB substrate.
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![Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)](https://www.abcam.com/ps/products/275/ab275960/Images/ab275960-6-benefits-of-recombinant-antibodies.png)
Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)