Antibodies, Proteins, Kits and Reagents for Life Science

271 382 ₸ Product size

100 µl 271 382 ₸

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Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR104H] to TBR2 / Eomes - BSA free
Suitable for: WB, IP, IHC-P, ICC
Reacts with: Human

Product name

Anti-TBR2 / Eomes antibody [BLR104H] - BSA free
See all TBR2 / Eomes primary antibodies
Description

Rabbit monoclonal [BLR104H] to TBR2 / Eomes - BSA free
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human TBR2/ Eomes aa 125-175. The exact sequence is proprietary. Genbank: NP_001265111.1
Database link: O95936
Positive control
- WB: NK-92 whole cell lysate. IP: NK-92 whole cell lysate. IHC-P: Human tonsil and non-Hodgkin's B-cell lymphoma tissue. ICC: NK-92 cells
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.80
Preservative: 0.09% Sodium azide
Constituent: 99.91% Borate buffered saline
Concentration information loading...
Purity

IgG fraction
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR104H
Isotype

IgG
Research areas

Immunoprecipitation - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)

Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from NK-92 cells was
prepared using NETN lysis buffer. ab275960 used for IP at 6 µl per reaction. For blotting immunoprecipitated TBR2 / Eomes, ab275960 was used at 1/1000 dilution.

Lane 1: ab275960 IP

Lane 2: Control IgG IP

Chemiluminescence with an exposure time of 10 seconds.
Western blot - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)

All lanes : Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960) at 1/1000 dilution

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 3 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate at 50 µg
Lane 4 : NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) whole cell lysate at 10 µg

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG

Predicted band size: 72 kDa

Exposure time: 75 seconds
Immunocytochemistry - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)

NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) cells stained for TBR2 / Eomes using ab275960 at 1/100 dilution in immunocytochemistry. Secondary antibody is a HRP-conjugated Goat anti-Rabbit IgG. DAB substrate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)

Formalin-fixed, paraffin-embedded non-Hodgkin's B-cell lymphoma tissue stained for TBR2 / Eomes using ab275960 at 1/100 dilution in immunohistochemical analysis. Secondary antibody is a HRP-conjugated Goat anti-Rabbit IgG. DAB substrate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for TBR2 / Eomes using ab275960 at 1/100 dilution in immunohistochemical analysis. Secondary antibody is a HRP-conjugated Goat anti-Rabbit IgG. DAB substrate.
Anti-TBR2 / Eomes antibody [BLR104H] - BSA free (ab275960)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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