Antibodies, Proteins, Kits and Reagents for Life Science

395 347 ₸ Product size

100 µl 395 347 ₸

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Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [E229] to p38 alpha/MAPK14
Suitable for: WB, ICC/IF, IP, Flow Cyt
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-p38 alpha/MAPK14 antibody [E229]
See all p38 alpha/MAPK14 primary antibodies
Description

Rabbit monoclonal [E229] to p38 alpha/MAPK14
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human p38 aa 150-250 (internal sequence). The exact sequence is proprietary.
Database link: Q16539
Positive control
- WB: Jurkat, C6, NIH/3T3 or HeLa whole cell lysate (ab150035). ICC/IF: NIH/3T3 cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Stable for 12 months at -20°C.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

E229
Isotype

IgG
Research areas

Western blot - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: p38 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab170099 observed at 40 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab170099 was shown to specifically react with p38 when p38 knockout samples were used. Wild-type and p38 knockout samples were subjected to SDS-PAGE. ab170099 and

ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)

Immunocytochemistry/ Immunofluorescence analysis of HeLa(Human epithelial cell line from cervix adenocarcinoma) cells labeling p38 with ab170099 at 1/250. Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077, an AlexaFluor®488 Goat anti-Rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab195889, an anti-alpha tubulin antibody [DM1A] microtubule marker (Alexa Fluor® 594) at 1/200. Nuclei counterstained with DAPI (blue).

Confocal image shows nuclear and cytoplasmic staining on HeLa cell line.
Western blot - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: p38 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab170099 observed at 40 kDa. Red - loading control, ab8245, observed at 37 kDa.

This western blot image is a comparison between ab170099 and a competitor's top cited rabbit polyclonal antibody.
Western blot - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)

Anti-p38 alpha/MAPK14 antibody [E229] (ab170099) at 1/5000 dilution (purified) + C6 cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 42 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)

All lanes : Anti-p38 alpha/MAPK14 antibody [E229] (ab170099) at 1/5000 dilution (purified)

Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 42 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)

All lanes : Anti-p38 alpha/MAPK14 antibody [E229] (ab170099) at 1/5000 dilution (purified)

Lane 1 : NIH/3T3 cell lysate
Lane 2 : 3T3-L1 cell lysate
Lane 3 : PC-12 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Lane 3 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 20 µg

Predicted band size: 42 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)

All lanes : Anti-p38 alpha/MAPK14 antibody [E229] (ab170099) at 1/5000 dilution (purified)

Lane 1 : MCF-7 cell lysate
Lane 2 : HEK293 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 42 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunoprecipitation - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)

ab170099 (purified) at 1/20 immunoprecipitating p38 in Jurkat whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM /TBST.
Flow Cytometry - Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)

Flow Cytometry analysis of HeLa cells labelling p38 with purified ab170099 at 1/40 (red). Cells were fixed with 4% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Anti-p38 alpha/MAPK14 antibody [E229] (ab170099)

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