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Epigenetics and Nuclear Signaling Histones HMGs

Anti-SRY/TDF antibody [OTI3C8] (ab140309)

Price and availability

274 732 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-SRY/TDF antibody [OTI3C8] (ab140309)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [OTI3C8] to SRY/TDF
  • Suitable for: WB, ICC/IF
  • Reacts with: Human, African green monkey
  • Isotype: IgG1

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Overview

  • Product name

    Anti-SRY/TDF antibody [OTI3C8]
    See all SRY/TDF primary antibodies
  • Description

    Mouse monoclonal [OTI3C8] to SRY/TDF
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human, African green monkey
    Predicted to work with: Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Recombinant full length protein corresponding to Human SRY/TDF aa 1-204.
    Sequence:

    MQSYASAMLSVFNSDDYSPAVQENIPALRRSSSFLCTESCNSKYQCETGE NSKGNVQDRVKRPMNAFIVW SRDQRRKMALENPRMRNSEISKQLGYQW KMLTEAEKWPFFQEAQKLQAMHREKYPNYKYRPRRKAKMLPK NCSLLP ADPASVLCSEVQLDNRLYRDDCTKATHSRMEHQLGHLPPINAASSPQQRD RYSHWTKL


    Database link: NP_003131
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Purchase matching WB positive control:Recombinant Human SRY/TDF protein
    • WB: HEK293T cells transfected with pCMV6-ENTRY SRY/TDF cDNA; ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY SRY/TDF.
  • General notes

    The clone number has been updated from 3C8 to OTI3C8, both clone numbers name the same clone.

     

    This product was previously labelled as SRY

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Purified from cell culture supernatant by affinity chromatography
  • Clonality

    Monoclonal
  • Clone number

    OTI3C8
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • HMGs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HMG Box
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Developmental Biology
    • Reproduction
    • Sex determination
    • Developmental Biology
    • Reproduction
    • Placental development

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-SRY/TDF antibody [OTI3C8] (ab140309)
    Immunocytochemistry/ Immunofluorescence - Anti-SRY/TDF antibody [OTI3C8] (ab140309)

    Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SRY/TDF, labelling SRY/TDF with ab140309 at 1/100 dilution.

  • Western blot - Anti-SRY/TDF antibody [OTI3C8] (ab140309)
    Western blot - Anti-SRY/TDF antibody [OTI3C8] (ab140309)
    All lanes : Anti-SRY/TDF antibody [OTI3C8] (ab140309) at 1/2000 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA.
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY SRY/TDF cDNA.

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 24 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: Flow Cyt, IHC-P, WB

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    Applications: WB

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