Anti-HMGB1 antibody - N-terminal (ab228624)
Key features and details
- Rabbit polyclonal to HMGB1 - N-terminal
- Suitable for: WB, IP, IHC-P, IHC-Fr, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-HMGB1 antibody - N-terminal
See all HMGB1 primary antibodies -
Description
Rabbit polyclonal to HMGB1 - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatHumanIHC-Fr MouseIHC-P MouseHumanIP HumanWB MouseRatHuman
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Immunogen
Synthetic peptide within Human HMGB1 (N terminal). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: P09429 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts; Mouse and rat brain tissue extracts. IHC-P: SkHep1 xenograft tissue; mouse urinary bladder and vein tissues. IHC-Fr: Adult mouse cerebellum tissue. ICC/IF: NTERA-2 cl.D1 [NT2/D1] cells; DIV9 rat E18 primary cortical neurons. IP: HEK-293T whole cell extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HMGB1 antibody - N-terminal (ab228624)All lanes : Anti-HMGB1 antibody - N-terminal (ab228624) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with HMGB1 shRNA, whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 25 kDa12% SDS-PAGE gel.
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Western blot - Anti-HMGB1 antibody - N-terminal (ab228624)All lanes : Anti-HMGB1 antibody - N-terminal (ab228624) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 25 kDa12% SDS-PAGE gel.
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Western blot - Anti-HMGB1 antibody - N-terminal (ab228624)Anti-HMGB1 antibody - N-terminal (ab228624) at 1/500 dilution + Mouse brain tissue extract at 50 µg
Predicted band size: 25 kDa12% SDS-PAGE gel.
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Western blot - Anti-HMGB1 antibody - N-terminal (ab228624)Anti-HMGB1 antibody - N-terminal (ab228624) at 1/500 dilution + Rat brain tissue extract at 50 µg
Predicted band size: 25 kDa12% SDS-PAGE gel.
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Immunohistochemistry (Frozen sections) - Anti-HMGB1 antibody - N-terminal (ab228624)Frozen sections of adult mouse cerebellum tissue stained for HMGB1 (green) using ab228624 at 1/250 dilution in immunohistochemical analysis.
Nuclear counterstain: Fluoroshield with DAPI (blue). NF-H, stained by an anti-NF-H antibody (red) at 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGB1 antibody - N-terminal (ab228624)Paraffin-embedded SkHep1 (human) xenograft tissue stained for HMGB1 using ab228624 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGB1 antibody - N-terminal (ab228624)Paraffin-embedded mouse urinary bladder tissue stained for HMGB1 using ab228624 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGB1 antibody - N-terminal (ab228624)Paraffin-embedded mouse vein tissue stained for HMGB1 using ab228624 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-HMGB1 antibody - N-terminal (ab228624)4% paraformaldehyde-fixed NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) cells stained for HMGB1 (green) using ab228624 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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Immunocytochemistry/ Immunofluorescence - Anti-HMGB1 antibody - N-terminal (ab228624)4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons stained for HMGB1 (green) using ab228624 at 1/500 dilution in ICC/IF.
Counterstain: beta Tubulin 3/Tuj1, a neuron cell marker, stained by an anti-beta Tubulin 3/Tuj1 antibody at 1/500 dilution (red).
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Immunoprecipitation - Anti-HMGB1 antibody - N-terminal (ab228624)HMGB1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg of ab228624. Western blot was performed from the immunoprecipitate using ab228624. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG instead of ab228624 in HEK-293T whole cell extract.
Lane 2: ab228624 IP in HEK-293T whole cell extract.
