Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SKP2 antibody [EPR3305(2)] (ab183039)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3305(2)] to SKP2
  • Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SKP2 antibody [EPR3305(2)]
    See all SKP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR3305(2)] to SKP2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: C6, NIH/3T3, F9, A549, MDA-MB-231, Jurkat, MCF7, HepG2, and NCCIT cell lysates. ICC: 293T cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SKP2 antibody [EPR3305(2)] (ab183039)
    Western blot - Anti-SKP2 antibody [EPR3305(2)] (ab183039)
    All lanes : Anti-SKP2 antibody [EPR3305(2)] (ab183039) at 1/200 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : SKP2 knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : MCF7 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 48 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab183039 observed at 52 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab183039 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in SKP2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SKP2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab183039 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/200 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-SKP2 antibody [EPR3305(2)] (ab183039)
    Immunocytochemistry/ Immunofluorescence - Anti-SKP2 antibody [EPR3305(2)] (ab183039)

    Immunofluorescenct analysis of 4% paraformaldehyde fixed 293T cells labeling SKP2 with ab183039 at 1/100 followed by Goat anti rabbit IgG(Alexa Fluor® 488) at 1/200 (green). Cells were counter stained with Dapi (blue).

  • Flow Cytometry - Anti-SKP2 antibody [EPR3305(2)] (ab183039)
    Flow Cytometry - Anti-SKP2 antibody [EPR3305(2)] (ab183039)

    Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling SKP2 with ab183039 at 1/70 followed by Goat anti rabbit IgG (FITC) at 1/150. Rabbit monoclonal IgG was used as Isotype control.

  • Western blot - Anti-SKP2 antibody [EPR3305(2)] (ab183039)
    Western blot - Anti-SKP2 antibody [EPR3305(2)] (ab183039)
    All lanes : Anti-SKP2 antibody [EPR3305(2)] (ab183039) at 1/200 dilution

    Lane 1 : NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
    Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
    Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
    Lane 4 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates with 5% NFDM/TBST
    Lane 5 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
    Lane 6 : A549 (Human lung carcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
    Lane 7 : F9 (Mouse embryonal carcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
    Lane 8 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates with 5% NFDM/TBST
    Lane 9 : C6 (Rat glial tumor glial cell) whole cell lysate with 5% NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 48 kDa


    Exposure time: 180 seconds


    We recommend to increase the amount of samples or decrease antibody dilution to get clear bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SKP2 antibody [EPR3305(2)] (ab183039)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SKP2 antibody [EPR3305(2)] (ab183039)

    immunohistochemical analysis of paraffin embedded Human kidney clear cell carcinoma tissue labeling SKP2 with ab183039 at 1/50 followed by secondary staining with Ready to use HRP Polymer for Rabbit IgG and counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-SKP2 antibody [EPR3305(2)] (ab183039)
    Anti-SKP2 antibody [EPR3305(2)] (ab183039)

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