Anti-SKP2 antibody (ab216687)
Key features and details
- Rabbit polyclonal to SKP2
- Suitable for: IHC-P, Flow Cyt, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SKP2 antibody
See all SKP2 primary antibodies -
Description
Rabbit polyclonal to SKP2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cyt, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human SKP2 aa 350 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q13309 -
Positive control
- Human thyroid carcinoma tissue; MCF-7 cells; HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SKP2 antibody (ab216687)Anti-SKP2 antibody (ab216687) at 1/500 dilution + 293T lysates
Secondary
Conjugated secondary antibody at 1/10000 dilution
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SKP2 antibody (ab216687)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thyroid carcinoma tissue labeling SKP2 with ab216687 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-SKP2 antibody (ab216687)Immunofluorescent analysis of MCF-7 cells labeling SKP2 with ab216687 at 1/500 dilution in PBS for two hours at 37°C, followed by Goat anti-rabbit IgG (H+L), Cy3 conjugated secondary antibody. The nucleus was stained with DAPI.
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Flow Cytometry - Anti-SKP2 antibody (ab216687)Flow cytometric analysis of HeLa cells labeling SKP2 with ab 216687 at 1/100 dilution for 30 minutes followed by a PE conjugated secondary antibody (green), compared to unstained cells (blue), secondary only (light blue), and an isotype control (orange).
