Antibodies, Proteins, Kits and Reagents for Life Science

254 630 ₸ Product size

100 µl 254 630 ₸

Order now and get it on Tuesday March 02, 2021

Anti-SKP2 antibody [EPR3306(2)] (ab124799)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3306(2)] to SKP2
Suitable for: WB, ICC/IF
Knockout validated
Reacts with: Human

Product name

Anti-SKP2 antibody [EPR3306(2)]
See all SKP2 primary antibodies
Description

Rabbit monoclonal [EPR3306(2)] to SKP2
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human SKP2 aa 150-250. The exact sequence is proprietary.
Positive control
- 293T, HeLa, SW480 and LnCaP cell lysates; HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3306(2)
Isotype

IgG
Research areas

Western blot - Anti-SKP2 antibody [EPR3306(2)] (ab124799)

All lanes : Anti-SKP2 antibody [EPR3306(2)] (ab124799) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : SKP2 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : MCF7 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 46, 48 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab124799 observed at 52 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab124799 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in SKP2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SKP2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab124799 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-SKP2 antibody [EPR3306(2)] (ab124799)

All lanes : Anti-SKP2 antibody [EPR3306(2)] (ab124799) at 1/1000 dilution

Lane 1 : 293T cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : SW480 cell lysates
Lane 4 : LnCaP cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 46, 48 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SKP2 antibody [EPR3306(2)] (ab124799)

ab124799, at a 1/50 dilution, staining SKP2 in HeLa cells by Immunofluorescence.
Anti-SKP2 antibody [EPR3306(2)] (ab124799)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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