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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin E3 Enzymes F Box E3 Ligase

Anti-Emi1 antibody [EPR15320] - BSA and Azide free (ab250788)

Anti-Emi1 antibody [EPR15320] - BSA and Azide free (ab250788)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15320] to Emi1 - BSA and Azide free
  • Suitable for: IHC-P, ICC, Flow Cyt (Intra), WB
  • Reacts with: Human

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Anti-Emi1 antibody [EMI1/1176] (ab215765)

Overview

  • Product name

    Anti-Emi1 antibody [EPR15320] - BSA and Azide free
    See all Emi1 primary antibodies
  • Description

    Rabbit monoclonal [EPR15320] to Emi1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC, Flow Cyt (Intra), WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250788 is the carrier-free version of ab184950.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15320
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • F Box E3 Ligase

Images

  • Immunocytochemistry - Anti-Emi1 antibody [EPR15320] - BSA and Azide free (ab250788)
    Immunocytochemistry - Anti-Emi1 antibody [EPR15320] - BSA and Azide free (ab250788)
    This data was developed using ab184950, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling Emi1 with ab184950 at 1/250 dilution. Goat anti-rabbit IgG (Alexa Fluor® 488) at 1/200 dilution was used as the secondary antibody. The slide on the right is counter stained with Dapi (blue).
  • Western blot - Anti-Emi1 antibody [EPR15320] - BSA and Azide free (ab250788)
    Western blot - Anti-Emi1 antibody [EPR15320] - BSA and Azide free (ab250788)
    All lanes : Anti-Emi1 antibody [EPR15320] (ab184950) at 1/5000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa



    This data was developed using ab184950, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody [EPR15320] - BSA and Azide free (ab250788)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody [EPR15320] - BSA and Azide free (ab250788)
    This data was developed using ab184950, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling Emi1 with ab184950 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-Emi1 antibody [EPR15320] - BSA and Azide free (ab250788)
    Flow Cytometry - Anti-Emi1 antibody [EPR15320] - BSA and Azide free (ab250788)
    This data was developed using ab184950, the same antibody clone in a different buffer formulation.Flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling Emi1 with ab184950 at 1/180 dilution (red). Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control (green).
  • Anti-Emi1 antibody [EPR15320] - BSA and Azide free (ab250788)
    Anti-Emi1 antibody [EPR15320] - BSA and Azide free (ab250788)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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