Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3198] to Cullin 4A/CUL-4A - BSA and Azide free
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free
    See all Cullin 4A/CUL-4A primary antibodies

  • Description

    Rabbit monoclonal [EPR3198] to Cullin 4A/CUL-4A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247585 is the carrier-free version of ab92554 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247585 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Cullin 4a, Cullin 4A/B

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)
    Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)
    All lanes : Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554) at 1/20000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : A375 cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 88 kDa



    This data was developed using ab92554, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)
    This data was developed using ab92554, the same antibody clone in a different buffer formulation.ab92554 at 1/100 dilution staining Cullin 4A/CUL-4A in Human breast carcinoma by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)
    This data was developed using ab92554, the same antibody clone in a different buffer formulation.ab92554 showing positive staining in Hepatocellular carcinoma tissue.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)
    This data was developed using ab92554, the same antibody clone in a different buffer formulation.ab92554 showing positive staining in Colonic adenocarcinoma tissue.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)
    This data was developed using ab92554, the same antibody clone in a different buffer formulation.ab92554 showing positive staining in Ovarian carcinoma tissue.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)
    Anti-Cullin 4A/CUL-4A antibody [EPR3198] - BSA and Azide free (ab247585)

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