Anti-FBXO21 antibody [EPR13163] (ab179818)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13163] to FBXO21
- Suitable for: IHC-P, WB, Flow Cyt, IP
- Reacts with: Human
Overview
-
Product name
Anti-FBXO21 antibody [EPR13163]
See all FBXO21 primary antibodies -
Description
Rabbit monoclonal [EPR13163] to FBXO21 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanIP HumanWB Human -
Immunogen
Recombinant fragment within Human FBXO21. The exact sequence is proprietary.
Database link: O94952 -
Positive control
- MCF-7, HeLa, HepG2 and U87-MG cell lysates; Human brain and kidney tissues; Hela cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13163 -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-FBXO21 antibody [EPR13163] (ab179818) at 1/10000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : U87-MG cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 72 kDa
-
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling FBXO21 with ab179818.
-
Flow cytometric analysis of permeabilized HeLa cells labeling FBXO21 with ab179818 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).
-
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling FBXO21 with ab179818 at 1/50 dilution.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling FBXO21 with ab179818 at 1/50 dilution.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-