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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin E3 Enzymes F Box E3 Ligase

Anti-FBXO21 antibody [EPR13163] (ab179818)

Price and availability

311 587 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-FBXO21 antibody [EPR13163] (ab179818)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13163] to FBXO21
  • Suitable for: IHC-P, WB, Flow Cyt, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-FBXO21 antibody [EPR13163]
    See all FBXO21 primary antibodies
  • Description

    Rabbit monoclonal [EPR13163] to FBXO21
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment within Human FBXO21. The exact sequence is proprietary.
    Database link: O94952

  • Positive control

    • MCF-7, HeLa, HepG2 and U87-MG cell lysates; Human brain and kidney tissues; Hela cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13163
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • F Box E3 Ligase
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • F-box

Images

  • Western blot - Anti-FBXO21 antibody [EPR13163] (ab179818)
    Western blot - Anti-FBXO21 antibody [EPR13163] (ab179818)
    All lanes : Anti-FBXO21 antibody [EPR13163] (ab179818) at 1/10000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : U87-MG cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 72 kDa

  • Immunoprecipitation - Anti-FBXO21 antibody [EPR13163] (ab179818)
    Immunoprecipitation - Anti-FBXO21 antibody [EPR13163] (ab179818)

    Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling FBXO21 with ab179818.

  • Flow Cytometry - Anti-FBXO21 antibody [EPR13163] (ab179818)
    Flow Cytometry - Anti-FBXO21 antibody [EPR13163] (ab179818)

    Flow cytometric analysis of permeabilized HeLa cells labeling FBXO21 with ab179818 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO21 antibody [EPR13163] (ab179818)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO21 antibody [EPR13163] (ab179818)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling FBXO21 with ab179818 at 1/50 dilution.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO21 antibody [EPR13163] (ab179818)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO21 antibody [EPR13163] (ab179818)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling FBXO21 with ab179818 at 1/50 dilution.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-FBXO21 antibody [EPR13163] (ab179818)
    Anti-FBXO21 antibody [EPR13163] (ab179818)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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