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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin E3 Enzymes F Box E3 Ligase

Anti-FBXO21 antibody [EPR13163] - BSA and Azide free (ab250120)

Anti-FBXO21 antibody [EPR13163] - BSA and Azide free (ab250120)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13163] to FBXO21 - BSA and Azide free
  • Suitable for: IHC-P, WB, IP, Flow Cyt
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-FBXO21 antibody [EPR13163] - BSA and Azide free
    See all FBXO21 primary antibodies
  • Description

    Rabbit monoclonal [EPR13163] to FBXO21 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human FBXO21. The exact sequence is proprietary.
    Database link: O94952

  • General notes

    Ab250120 is the carrier-free version of ab179818. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250120 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13163
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • F Box E3 Ligase
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • F-box

Images

  • Western blot - Anti-FBXO21 antibody [EPR13163] - BSA and Azide free (ab250120)
    Western blot - Anti-FBXO21 antibody [EPR13163] - BSA and Azide free (ab250120)
    All lanes : Anti-FBXO21 antibody [EPR13163] (ab179818) at 1/10000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : U-87 MG cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 72 kDa



    This data was developed using ab179818, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-FBXO21 antibody [EPR13163] - BSA and Azide free (ab250120)
    Immunoprecipitation - Anti-FBXO21 antibody [EPR13163] - BSA and Azide free (ab250120)
    This data was developed using ab179818, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling FBXO21 with ab179818.
  • Flow Cytometry - Anti-FBXO21 antibody [EPR13163] - BSA and Azide free (ab250120)
    Flow Cytometry - Anti-FBXO21 antibody [EPR13163] - BSA and Azide free (ab250120)
    This data was developed using ab179818, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized HeLa cells labeling FBXO21 with ab179818 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO21 antibody [EPR13163] - BSA and Azide free (ab250120)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO21 antibody [EPR13163] - BSA and Azide free (ab250120)
    This data was developed using ab179818, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling FBXO21 with ab179818 at 1/50 dilution. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO21 antibody [EPR13163] - BSA and Azide free (ab250120)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO21 antibody [EPR13163] - BSA and Azide free (ab250120)
    This data was developed using ab179818, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling FBXO21 with ab179818 at 1/50 dilution. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Anti-FBXO21 antibody [EPR13163] - BSA and Azide free (ab250120)
    Anti-FBXO21 antibody [EPR13163] - BSA and Azide free (ab250120)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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