Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STAT4 antibody [EP1900Y] - BSA and Azide free (ab247366)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1900Y] to STAT4 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC, IHC-P, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-STAT4 antibody [EP1900Y] - BSA and Azide free
    See all STAT4 primary antibodies

  • Description

    Rabbit monoclonal [EP1900Y] to STAT4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC, IHC-P, WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MOLT-4 and Daudi cell lysates. IHC-P: Human tonsil and Human ovarian cancer tissue sections. ICC: HeLa cells. Flow Cyt (intra): K562 cells. IP: HeLa whole cell lysate.

  • General notes

    ab247366 is the carrier-free version of ab68156.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Images

  • Western blot - Anti-STAT4 antibody [EP1900Y] - BSA and Azide free (ab247366)
    Western blot - Anti-STAT4 antibody [EP1900Y] - BSA and Azide free (ab247366)
    All lanes : Anti-STAT4 antibody [EP1900Y] (ab68156) at 1/1000 dilution

    Lane 1 : Daudi cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 86 kDa
    Observed band size: 86 kDa
    Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab68156, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-STAT4 antibody [EP1900Y] - BSA and Azide free (ab247366)
    Immunocytochemistry - Anti-STAT4 antibody [EP1900Y] - BSA and Azide free (ab247366)
    This data was developed using the same antibody clone in a different buffer formulation (ab68156).
    Immunocytochemistry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling STAT4 with purified ab68156 at 1/50 dilution (9 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.16 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
    Negative control: PBS instead of the primary antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT4 antibody [EP1900Y] - BSA and Azide free (ab247366)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT4 antibody [EP1900Y] - BSA and Azide free (ab247366)
    This data was developed using ab68156, the same antibody clone in a different buffer formulation.ab68156, at 1/100 dilution, staining STAT4 in paraffin embedded human tonsil tissue sections by Immunohistochemistry Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-STAT4 antibody [EP1900Y] - BSA and Azide free (ab247366)
    Immunoprecipitation - Anti-STAT4 antibody [EP1900Y] - BSA and Azide free (ab247366)

    This data was developed using ab68156, the same antibody clone in a different buffer formulation.
    Purified ab68156 at 1/30 dilution (2µg) immunoprecipitating STAT4 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab68156 + HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68156 in HeLa whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 86 kDa

  • Flow Cytometry - Anti-STAT4 antibody [EP1900Y] - BSA and Azide free (ab247366)
    Flow Cytometry - Anti-STAT4 antibody [EP1900Y] - BSA and Azide free (ab247366)
    This data was developed using ab68156, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of K562 (Human chronic myelogenous leukemia lymphoblast) cells labeling STAT4 with purified ab68156 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
  • Anti-STAT4 antibody [EP1900Y] - BSA and Azide free (ab247366)
    Anti-STAT4 antibody [EP1900Y] - BSA and Azide free (ab247366)

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