Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)
![Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)](https://www.abcam.com/ps/products/179/ab179831/Images/ab179831-208219-anti-smc4-antibody-epr13366-c-terminal-western-blot.jpg "Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13366] to SMC4 - C-terminal
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-SMC4 antibody [EPR13366] - C-terminal
See all SMC4 primary antibodies -
Description
Rabbit monoclonal [EPR13366] to SMC4 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SMC4 aa 1200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9NTJ3 -
Positive control
- Ramos, 293T, HeLa and Raji cell lysates. HeLa and Raji cells.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13366 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)All lanes : Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831) at 1/1000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 147 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)](https://www.abcam.com/ps/products/179/ab179831/Images/ab179831-208220-anti-smc4-antibody-epr13366-c-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)Immunofluorescent analysis of HeLa cells labeling SMC4 with ab179831 at 1/50 dilution.
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![Flow Cytometry - Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)](https://www.abcam.com/ps/products/179/ab179831/Images/ab179831-208221-anti-smc4-antibody-epr13366-c-terminal-flow-cytometry.jpg)
Flow Cytometry - Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)Flow cytometric analysis of permeabilized Raji cells using ab179831 at 1/10 (red) or a rabbit IgG negative (green).
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![Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)](https://www.abcam.com/ps/products/179/ab179831/Images/ab179831-4-benefits-of-recombinant-antibodies.png)
Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)
