Anti-SMC4 antibody - N-terminal (ab229213)
Key features and details
- Rabbit polyclonal to SMC4 - N-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-SMC4 antibody - N-terminal
See all SMC4 primary antibodies -
Description
Rabbit polyclonal to SMC4 - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Recombinant fragment within Human SMC4 (N terminal). The exact sequence is proprietary.
Database link: Q9NTJ3 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell lysates. IP: HEK-293T whole cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-SMC4 antibody - N-terminal (ab229213)All lanes : Anti-SMC4 antibody - N-terminal (ab229213) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 147 kDa5% SDS-PAGE gel.
-
Immunoprecipitation - Anti-SMC4 antibody - N-terminal (ab229213)SMC4 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg of ab229213. Western blot was performed from the immunoprecipitate using ab229213. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HEK-293T whole cell extract (Input).
Lane 2: Control IgG instead of ab229213 in HEK-293T whole cell extract.
Lane 3: ab229213 IP in HEK-293T whole cell extract.
