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Anti-COX5B antibody [16H12H9] (ab110263)

Key features and details

  • Mouse monoclonal [16H12H9] to COX5B
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-COX5B antibody [16H12H9]
    See all COX5B primary antibodies

  • Description

    Mouse monoclonal [16H12H9] to COX5B

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human

  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • Isolated mitochondria from Human heart, Bovine heart, Rat heart, and Mouse heart

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Images

  • Western blot - Anti-COX5B antibody [16H12H9] (ab110263)
    Western blot - Anti-COX5B antibody [16H12H9] (ab110263)
    All lanes : Anti-COX5B antibody [16H12H9] (ab110263) at 1 µg/ml

    Lane 1 : Human heart mitochondria at 5 µg
    Lane 2 : Bovine heart mitochondria at 1 µg
    Lane 3 : Rat heart mitochondria at 10 µg
    Lane 4 : Mouse heart mitochondria at 10 µg

    Predicted band size: 14 kDa

  • Flow Cytometry - Anti-COX5B antibody [16H12H9] (ab110263)
    Flow Cytometry - Anti-COX5B antibody [16H12H9] (ab110263)
    Overlay histogram showing HepG2 cells stained with ab110263 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110263, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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