Anti-CD43 antibody [SPN/1766R] (ab218523)
![Anti-CD43 antibody [SPN/1766R] (ab218523)](https://www.abcam.com/ps/products/218/ab218523/Images/ab218523-456468-anti-cd43-antibody-spn-1766r-immunocytochemistry-k562-human.jpg "Anti-CD43 antibody [SPN/1766R] (ab218523)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SPN/1766R] to CD43
- Suitable for: WB, ICC, Flow Cyt, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-CD43 antibody [SPN/1766R]
See all CD43 primary antibodies -
Description
Rabbit monoclonal [SPN/1766R] to CD43 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human CD43 aa 1-400.
Database link: P16150 -
Positive control
- IHC-P: Human tonsil tissue; WB: K562 cell lysate; ICC and Flow Cyt: K562 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
SPN/1766R -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry - Anti-CD43 antibody [SPN/1766R] (ab218523)
Immunocytochemistry analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CD43 with ab218523 at 2 µg/mL followed by Goat anti-Rabbit- IgG-CF488 (green). The nuclear counterstain is NucSpot (red).
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![Flow Cytometry - Anti-CD43 antibody [SPN/1766R] (ab218523)](https://www.abcam.com/ps/products/218/ab218523/Images/ab218523-456469-anti-cd43-antibody-spn-1766r-flow-cytometry-k562-human.jpg)
Flow Cytometry - Anti-CD43 antibody [SPN/1766R] (ab218523)Flow Cytometry analysis of PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CD43 with ab218523 at 2 µg/106 cells followed by Goat anti-Rabbit- IgG-CF488 (Blue); Isotype Control (Red).
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![Western blot - Anti-CD43 antibody [SPN/1766R] (ab218523)](https://www.abcam.com/ps/products/218/ab218523/Images/ab218523-456470-anti-cd43-antibody-spn-1766r-western-blot-k562-human.jpg)
Western blot - Anti-CD43 antibody [SPN/1766R] (ab218523)Anti-CD43 antibody [SPN/1766R] (ab218523) at 2 µg/ml + K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Predicted band size: 40 kDaCD43 protein has multiple sites of glycosylation which increases the apparent molecular weight in Western blot.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SPN/1766R] (ab218523)](https://www.abcam.com/ps/products/218/ab218523/Images/ab218523-274004-anti-cd43-antibody-spn-1766r-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SPN/1766R] (ab218523)Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human tonsil tissue labeling CD43 with ab218523 at 1 μg/ml.
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![Anti-CD43 antibody [SPN/1766R] (ab218523)](https://www.abcam.com/ps/products/218/ab218523/Images/ab218523-2-benefits-of-recombinant-antibodies.png)
Anti-CD43 antibody [SPN/1766R] (ab218523)
