HRP Anti-EpCAM antibody [EPR677(2)] (ab199521)
![HRP Anti-EpCAM antibody [EPR677(2)] (ab199521)](https://www.abcam.com/ps/products/199/ab199521/Images/ab199521-246916-anti-epcam-antibody-epr6772-hrp-western-blot.jpg "HRP Anti-EpCAM antibody [EPR677(2)] (ab199521)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR677(2)] to EpCAM
- Suitable for: WB, IHC-P
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-EpCAM antibody [EPR677(2)]
See all EpCAM primary antibodies -
Description
HRP Rabbit monoclonal [EPR677(2)] to EpCAM -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HT-29 whole cell lysate. IHC-P: normal human colon tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR677(2) -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-EpCAM antibody [EPR677(2)] (ab199521)HRP Anti-EpCAM antibody [EPR677(2)] (ab199521) at 1/2500 dilution +
HT-29 whole cell lysate (ab3952) at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab199521 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-EpCAM antibody [EPR677(2)] (ab199521)](https://www.abcam.com/ps/products/199/ab199521/Images/ab199521-244545-anti-epcam-antibody-epr6772-hrp-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-EpCAM antibody [EPR677(2)] (ab199521)IHC image of EpCAM staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab199521 at 1/100 dilution for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![HRP Anti-EpCAM antibody [EPR677(2)] (ab199521)](https://www.abcam.com/ps/products/199/ab199521/Images/ab199521-4-benefits-of-recombinant-antibodies.png)
HRP Anti-EpCAM antibody [EPR677(2)] (ab199521)
