Anti-LMO 4 antibody [20F8] - BSA and Azide free (ab255746)
![Anti-LMO 4 antibody [20F8] - BSA and Azide free (ab255746)](https://www.abcam.com/ps/products/255/ab255746/Images/ab255746-388761-RatmonoclonalLMO420F8westernblothap1t47dembryohumanmouse.jpg "Anti-LMO 4 antibody [20F8] - BSA and Azide free (ab255746)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [20F8] to LMO 4 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-LMO 4 antibody [20F8] - BSA and Azide free
See all LMO 4 primary antibodies -
Description
Rat monoclonal [20F8] to LMO 4 - BSA and Azide free -
Host species
Rat -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Mouse LMO 4. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P61969 -
Positive control
- WB: Wild-type HAP1 whole cell lysate. Mouse embryo tissue lysate. T47D whole cell lysate. IHC-P: Mouse and rat hippocampus tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab255746 is the carrier-free version of ab243139. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
20F8 -
Isotype
IgG2a
Images
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Western blot - Anti-LMO 4 antibody [20F8] - BSA and Azide free (ab255746)All lanes : Anti-LMO 4 antibody [20F8] (ab243139) at 1.354 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : T47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate
Lane 3 : Mouse embryo tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilutionBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1,2: 15 secs; Lane 3: 3 secs
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243139).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO 4 antibody [20F8] - BSA and Azide free (ab255746)](https://www.abcam.com/ps/products/255/ab255746/Images/ab255746-388759-RatmonoclonalLMO420F8immunohistochemistryhippocampusmouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO 4 antibody [20F8] - BSA and Azide free (ab255746)Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling LMO 4 with ab243139 at 1/1000 dilution, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on mouse hippocampus tissue is observed. The section was incubated with ab243139 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243139).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO 4 antibody [20F8] - BSA and Azide free (ab255746)](https://www.abcam.com/ps/products/255/ab255746/Images/ab255746-388757-RatmonoclonalLMO420F8immunohistochemistryhippocampusrat.png)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO 4 antibody [20F8] - BSA and Azide free (ab255746)Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling LMO 4 with ab243139 at 1/1000 dilution, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on rat hippocampus tissue is observed. The section was incubated with ab243139 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243139).
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![Anti-LMO 4 antibody [20F8] - BSA and Azide free (ab255746)](https://www.abcam.com/ps/products/255/ab255746/Images/ab255746-4-benefits-of-recombinant-antibodies.png)
Anti-LMO 4 antibody [20F8] - BSA and Azide free (ab255746)