Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP350] to Lysozyme - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Lysozyme antibody [SP350] - BSA and Azide free
    See all Lysozyme primary antibodies

  • Description

    Rabbit monoclonal [SP350] to Lysozyme - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Lysozyme (C terminal). The exact sequence is proprietary.
    Database link: P61626

  • Positive control

    • WB: HAP1, HepG2 and HL-60 whole cell lysate. IHC-P: Human tonsil, lung, liver, spleen, stomach adenocarcinoma and pancreatic adenocarcinoma tissues. Flow Cyt: HL-60 cells.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    ab242430 is the carrier-free version of ab227708. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab242430 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)
    Western blot - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)
    All lanes : Anti-Lysozyme antibody [SP350] - C-terminal (ab227708) at 1/400 dilution

    Lane 1 : Wild-type THP1 cell lysate
    Lane 2 : LYZ knockout THP1 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 16 kDa
    Observed band size: 16 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab227708).

    Lanes 1 - 4: Merged signal (red and green). Green - ab227708 observed at 16 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab227708 was shown to react with Lysozyme in wild-type THP1 cells in Western blot with loss of signal observed in LYZ knockout sample.Wild-type THP1 and LYZ knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab227708 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 400 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)

    Formalin-fixed, paraffin-embedded human spleen tissue stained for Lysozyme using ab227708 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227708).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)

    Formalin-fixed, paraffin-embedded human liver tissue stained for Lysozyme using ab227708 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227708).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)

    Formalin-fixed, paraffin-embedded human lung tissue stained for Lysozyme using ab227708 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227708).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)

    Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Lysozyme using ab227708 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227708).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)

    Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Lysozyme using ab227708 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227708).

  • Flow Cytometry - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)
    Flow Cytometry - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)

    Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cell line labeling Lysozyme with ab227708 at 1/100 dilution (green) compared with a Rabbit IgG negative control (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227708).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lysozyme using ab227708 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227708).

  • Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)
    Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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