Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Lysozyme antibody [SP350] - C-terminal (ab227708)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP350] to Lysozyme - C-terminal
Suitable for: WB, Flow Cyt, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-Lysozyme antibody [SP350] - C-terminal
See all Lysozyme primary antibodies
Description

Rabbit monoclonal [SP350] to Lysozyme - C-terminal
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human Lysozyme (C terminal).
Database link: P61626
Positive control
- WB: THP-1, HepG2 and HL-60 whole cell lysate. IHC-P: Human tonsil, lung, liver, spleen, stomach adenocarcinoma and pancreatic adenocarcinoma tissues. Flow Cyt: HL-60 cells.
General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

SP350
Isotype

IgG
Research areas

Western blot - Anti-Lysozyme antibody [SP350] - C-terminal (ab227708)

All lanes : Anti-Lysozyme antibody [SP350] - C-terminal (ab227708) at 1/400 dilution

Lane 1 : Wild-type THP1 cell lysate
Lane 2 : LYZ knockout THP1 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 16 kDa
Observed band size: 16 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab227708 observed at 16 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab227708 was shown to react with Lysozyme in wild-type THP1 cells in Western blot with loss of signal observed in LYZ knockout sample.Wild-type THP1 and LYZ knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab227708 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 400 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - C-terminal (ab227708)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lysozyme using ab227708 at 1/100 dilution in immunohistochemical analysis.
Flow Cytometry - Anti-Lysozyme antibody [SP350] - C-terminal (ab227708)

Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cell line labeling Lysozyme with ab227708 at 1/100 dilution (green) compared with a Rabbit IgG negative control (blue).
Western blot - Anti-Lysozyme antibody [SP350] - C-terminal (ab227708)

Anti-Lysozyme antibody [SP350] - C-terminal (ab227708) at 1/400 dilution + HL-60 (human promyelocytic leukemia cell line) cell lysate

Predicted band size: 16 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - C-terminal (ab227708)

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Lysozyme using ab227708 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - C-terminal (ab227708)

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Lysozyme using ab227708 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - C-terminal (ab227708)

Formalin-fixed, paraffin-embedded human lung tissue stained for Lysozyme using ab227708 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - C-terminal (ab227708)

Formalin-fixed, paraffin-embedded human liver tissue stained for Lysozyme using ab227708 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - C-terminal (ab227708)

Formalin-fixed, paraffin-embedded human spleen tissue stained for Lysozyme using ab227708 at 1/100 dilution in immunohistochemical analysis.
Anti-Lysozyme antibody [SP350] - C-terminal (ab227708)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com