Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD43 antibody [EPR21903] (ab235452)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21903] to CD43
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-CD43 antibody [EPR21903]
    See all CD43 primary antibodies

  • Description

    Rabbit monoclonal [EPR21903] to CD43

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HL-60, Ramos, IM-9 anf THP-1 whole cell lysates; Human colon and lung lysates. IHC-P: Human tonsil and B cell lymphoma tissue. ICC/IF: IM-9 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR21903] (ab235452)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR21903] (ab235452)

    Immunohistochemical analysis of paraffin-embedded human B cell lymphoma tissue labeling CD43 with ab235452 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on human B cell lymphoma (PMID: 2794085) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR21903] (ab235452)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR21903] (ab235452)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD43 with ab235452 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in the T cell zone and scattered T cells in the germinal center (PMID: 2794085) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

     

  • Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [EPR21903] (ab235452)
    Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [EPR21903] (ab235452)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized IM-9 (human multiple myeloma B lymphoblast cell line) cells labeling CD43 with ab235452 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on the IM-9 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red)

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-CD43 antibody [EPR21903] (ab235452)
    Western blot - Anti-CD43 antibody [EPR21903] (ab235452)
    All lanes : Anti-CD43 antibody [EPR21903] (ab235452) at 1/1000 dilution

    Lane 1 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
    Lane 2 : Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate
    Lane 3 : IM-9 (human multiple myeloma B lymphoblast cell line) whole cell lysate
    Lane 4 : THP-1 (human monocytic leukemia cell line0 whole cell lysate
    Lane 5 : Human colon lysate
    Lane 6 : Human lung lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 40 kDa
    Observed band size: 100-150,20,50-60 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1-4: 15 seconds; Lanes 5 & 6: 3 minutes.

    The expression profile observed is consistent with what has been described in the literature (PMIDs: 2794085, 23987472 and 7683406). We also observe additional bands at or below 20 kDa in some cell lines, which could be due to  proteolysitic cleavage of the protein (PMIDs: 18586676 and 15540986).

  • Anti-CD43 antibody [EPR21903] (ab235452)
    Anti-CD43 antibody [EPR21903] (ab235452)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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