Anti-RPA32/RPA2 antibody (ab10359)
Key features and details
- Rabbit polyclonal to RPA32/RPA2
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RPA32/RPA2 antibody
See all RPA32/RPA2 primary antibodies -
Description
Rabbit polyclonal to RPA32/RPA2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Bat, Elephant
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Immunogen
Immunogen was a synthetic peptide, which represented a portion of the C-terminus of human replication protein A2, 32 kDa subunit (LocusLink ID 6118).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RPA32/RPA2 antibody (ab10359)All lanes : Anti-RPA32/RPA2 antibody (ab10359) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : Mouse TCMK-1 whole cell lysate
Lane 5 : Mouse NIH 3T3 whole cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 32 kDa
Exposure time: 10 seconds
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Immunoprecipitation - Anti-RPA32/RPA2 antibody (ab10359)HeLa whole cell lysate cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-).
Affinity purified rabbit anti-RPA32 antibody (3 µg) and rabbit anti-Phospho RPA32 antibody (1/100 dilution) were used.
Detection: Chemiluminescence
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody (ab10359)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling RPA32/RPA2 with ab10359 at 1/5000 (0.2µg/ml). Detection: DAB.
