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Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (ab257327)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SPN/2049R] to CD43 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD43 antibody [SPN/2049R] - BSA and Azide free
    See all CD43 primary antibodies

  • Description

    Rabbit monoclonal [SPN/2049R] to CD43 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human CD43.
    Database link: P16150

  • Positive control

    • WB: K562 cell lysate. IHC-P: Human tonsil tissue. ICC/IF: K562 cells. Flow Cyt: K562 cells.

  • General notes

    ab257327 is a PBS only version of ab257316.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (ab257327)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (ab257327)

    Formalin-fixed, paraffin-embedded human tonsil tissue labeling CD43 using ab257316 at 2 μg/ml in immunohistochemical analysis.

    Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

    This data was produced with ab257316, the same antibody in a different formulation with BSA and Azide.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (ab257327)
    Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (ab257327)

    K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CD43 using ab257316 at 2 μg/ml, followed by Goat anti-Rabbit IgG CF488 (green) in ICC/IF.

    The nuclear counter stain is NucSpot (red).

    This data was produced with ab257316, the same antibody in a different formulation with BSA and Azide.

  • Western blot - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (ab257327)
    Western blot - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (ab257327)
    Anti-CD43 antibody [SPN/2049R] (ab257316) at 2 µg/ml + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate

    Predicted band size: 40 kDa



    This data was produced with ab257316, the same antibody in a different formulation with BSA and Azide.

  • Flow Cytometry - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (ab257327)
    Flow Cytometry - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (ab257327)

    PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CD43 using ab257316, followed by Goat anti-Rabbit IgG CF488 (blue) as compared with an isotype control (red).

    This data was produced with ab257316, the same antibody in a different formulation with BSA and Azide.

  • Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (ab257327)
    Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (ab257327)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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