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Anti-CD43 antibody [SPN/3388] - BSA and Azide free (ab257326)

Key features and details

  • Mouse monoclonal [SPN/3388] to CD43 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-CD43 antibody [SPN/3388] - BSA and Azide free
    See all CD43 primary antibodies

  • Description

    Mouse monoclonal [SPN/3388] to CD43 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human CD43.
    Database link: P16150

  • Positive control

    • IHC-P: Human spleen tissue. ICC/IF: K562 cells. Flow Cyt: K562 cells.

  • General notes

    ab257326 is a PBS only version of ab257315.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SPN/3388] - BSA and Azide free (ab257326)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SPN/3388] - BSA and Azide free (ab257326)

    Formalin-fixed, Paraffin-embedded human spleeen tissue labeling CD43 using ab257315 at 2 μg/ml in immunohistochemical analysis.

    Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

    This data was produced with ab257315, the same antibody in a different formulation with BSA and Azide.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [SPN/3388] - BSA and Azide free (ab257326)
    Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [SPN/3388] - BSA and Azide free (ab257326)

    K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CD43 using ab257315 at 2 μg/ml (green) in ICC/IF. 

    The nuclear counterstain is NucSpot Live 650 (red).

    This data was produced with ab257315, the same antibody in a different formulation with BSA and Azide.

  • Flow Cytometry - Anti-CD43 antibody [SPN/3388] - BSA and Azide free (ab257326)
    Flow Cytometry - Anti-CD43 antibody [SPN/3388] - BSA and Azide free (ab257326)

    Paraformadehyde-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CD43 using ab257315 at 2 μg / 106 cells (blue) as compared to an isotype control (red) in flow cytometric analysis.

    This data was produced with ab257315, the same antibody in a different formulation with BSA and Azide.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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