Anti-Von Willebrand Factor antibody [EPR18569-105] (ab189500)
![Anti-Von Willebrand Factor antibody [EPR18569-105] (ab189500)](https://www.abcam.com/ps/products/189/ab189500/Images/ab189500-293402-anti-von-willebrand-factor-antibody-epr18569-105-western-blot.jpg "Anti-Von Willebrand Factor antibody [EPR18569-105] (ab189500)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18569-105] to Von Willebrand Factor
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Von Willebrand Factor antibody [EPR18569-105]
See all Von Willebrand Factor primary antibodies -
Description
Rabbit monoclonal [EPR18569-105] to Von Willebrand Factor -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human and mouse plasma and liver lysates; Hepa1-6 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18569-105 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Von Willebrand Factor antibody [EPR18569-105] (ab189500)All lanes : Anti-Von Willebrand Factor antibody [EPR18569-105] (ab189500) at 1/1000 dilution
Lane 1 : Human liver lysate
Lane 2 : Human plasma
Lane 3 : Hep1-6 (mouse hepatoma epithelial cell line) whole cell lysate
Lane 4 : Mouse plasma
Lane 5 : Mouse liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 309 kDa
Observed band size: 309,90 kDa why is the actual band size different from the predicted?Exposure times: Lanes 1,2: 3 minutes; Lane 3: 1 minute; Lane 4,5: 15 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular masses are consistent with what has been described in the literature (PMID: 8204880; PMID: 18344423).
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![Anti-Von Willebrand Factor antibody [EPR18569-105] (ab189500)](https://www.abcam.com/ps/products/189/ab189500/Images/ab189500-3-benefits-of-recombinant-antibodies.png)
Anti-Von Willebrand Factor antibody [EPR18569-105] (ab189500)
