Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823)
![Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823)](https://www.abcam.com/ps/products/219/ab219823/Images/ab219823-286096-anti-mt-cyb-antibody-5b3-6e3-n-terminal-western-blot.jpg "Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823)")
Key features and details
- Mouse monoclonal [5B3-6E3] to MT-CYB - N-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-MT-CYB antibody [5B3-6E3] - N-terminal
See all MT-CYB primary antibodies -
Description
Mouse monoclonal [5B3-6E3] to MT-CYB - N-terminal -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human MT-CYB (N terminal).
Database link: P00156 -
Positive control
- WB: Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn); Whole cell extract of cultured normal control human dermal fibroblasts neonatal (HDFn).
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General notes
Western blot advice:
Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 0.36% HEPES, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Protein L purified -
Purification notes
Purified from hybridoma cell culture supernatant by Protein L affinity chromatography from fetal bovine serum containing medium (Protein L does not bind bovine IgG). -
Clonality
Monoclonal -
Clone number
5B3-6E3 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823)All lanes : Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823) at 0.1 µg/ml
Lane 1 : Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn)
Lane 2 : Mitochondria from HDFn cells depleted of mtDNA by long-term proliferation in the presence of ethidium bromide
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labeled Goat-anti-mouse IgG
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?Mitochondrial proteins solubilized in 2% SDS were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.
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![Western blot - Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823)](https://www.abcam.com/ps/products/219/ab219823/Images/ab219823-286098-anti-mt-cyb-antibody-5b3-6e3-n-terminal-western-blot.jpg)
Western blot - Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823)All lanes : Anti-MT-CYB antibody [5B3-6E3] - N-terminal (ab219823) at 2 µg/ml
Lane 1 : Whole cell extract of cultured normal control human dermal fibroblasts neonatal (HDFn)
Lane 2 : Whole cell extract HDFn-Rho0 cells depleted of mtDNA by long-term culture in the presence of ethidium bromide
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP-labeled Goat-anti-mouse IgG
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?Proteins were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.
