Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 107 212 ₸

Order now and get it on Tuesday March 02, 2021

Anti-BMAL1 antibody [EPR23696-22] (ab230822)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23696-22] to BMAL1
Suitable for: IP, IHC-P, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-BMAL1 antibody [EPR23696-22]
See all BMAL1 primary antibodies
Description

Rabbit monoclonal [EPR23696-22] to BMAL1
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat
IP	Mouse Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: NIH/3T3, Rat liver, PC-3 lysates. IHC-P: Mouse cardiac muscle, Mouse hippocampus, Rat pancreas tissues. IP: PC-3 and NIH/3T3 cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23696-22
Isotype

IgG
Research areas

Western blot - Anti-BMAL1 antibody [EPR23696-22] (ab230822)

All lanes : Anti-BMAL1 antibody [EPR23696-22] (ab230822) at 1/1000 dilution

Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 20
Lane 2 : Rat liver tissue lysate 20

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

Predicted band size: 68 kDa
Observed band size: 75 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST The molecular weight observed is consistent with what has been described in the literature (PMID: 28332504). Exposure time: 70 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMAL1 antibody [EPR23696-22] (ab230822)

Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling BMAL1 with ab230822 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in rat pancreas (PMID: 29396463). The section was incubated with ab230822 for 20 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Antigen retrieval was heat mediated with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Immunoprecipitation - Anti-BMAL1 antibody [EPR23696-22] (ab230822)

ab230822 at 1/30 (2μg in 0.35mg lysates) immunoprecipitating BMAL1 in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate.

Lane 1 (input): NIH/3T3 whole cell lysate (10µg)

Lane 2 (+): ab230822 + NIH/3T3 whole cell lysate.

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab230822 in NIH/3T3 whole cell lysate.

For western blotting, ab230822 at 1/1000 dilution (0.58 μg/mL) and ab131366 VeriBlot for IP (HRP) at 1/5000 was used for detection.

Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

Fresh lysates were used in this IP.
Western blot - Anti-BMAL1 antibody [EPR23696-22] (ab230822)

Anti-BMAL1 antibody [EPR23696-22] (ab230822) at 1/1000 dilution + PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate 20 at 20 µg

Secondary
VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

Predicted band size: 68 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST The molecular weight observed is consistent with what has been described in the literature (PMID: 28332504). Exposure time: 70 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMAL1 antibody [EPR23696-22] (ab230822)

Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling BMAL1 with ab230822 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in mouse hippocampus (PMID: 20382135). The section was incubated with ab230822 for 20 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Antigen retrieval was heat mediated with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Immunoprecipitation - Anti-BMAL1 antibody [EPR23696-22] (ab230822)

ab230822 at 1/30 (2μg in 0.35mg lysates) immunoprecipitating BMAL1 in PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate.

Lane 1 (input): PC-3 whole cell lysate (10µg)

Lane 2 (+): ab230822 + PC-3 whole cell lysate.

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab230822 in PC-3 whole cell lysate.

For western blotting, ab230822 at 1/1000 dilution (0.58 μg/mL) and ab131366 VeriBlot for IP (HRP) at 1/5000 was used for detection.

Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

Fresh lysates were used in this IP.
The molecular weight observed is consistent with what has been described in the literature (PMID: 28332504).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMAL1 antibody [EPR23696-22] (ab230822)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling BMAL1 with ab230822 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in mouse cardiac muscle (PMID: 10760301). The section was incubated with ab230822 for 20 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Antigen retrieval was heat mediated with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Anti-BMAL1 antibody [EPR23696-22] (ab230822)

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