Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BMAL1 antibody [EPR23696-22] (ab230822)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23696-22] to BMAL1
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-BMAL1 antibody [EPR23696-22]
    See all BMAL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23696-22] to BMAL1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    IP
    Mouse
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NIH/3T3, Rat liver, PC-3 lysates. IHC-P: Mouse cardiac muscle, Mouse hippocampus, Rat pancreas tissues. IP: PC-3 and NIH/3T3 cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-BMAL1 antibody [EPR23696-22] (ab230822)
    Western blot - Anti-BMAL1 antibody [EPR23696-22] (ab230822)
    All lanes : Anti-BMAL1 antibody [EPR23696-22] (ab230822) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 20
    Lane 2 : Rat liver tissue lysate 20

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 68 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST The molecular weight observed is consistent with what has been described in the literature (PMID: 28332504).  Exposure time: 70 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMAL1 antibody [EPR23696-22] (ab230822)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMAL1 antibody [EPR23696-22] (ab230822)

    Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling BMAL1 with ab230822 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in rat pancreas (PMID: 29396463). The section was incubated with ab230822 for 20 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Antigen retrieval was heat mediated with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

  • Immunoprecipitation - Anti-BMAL1 antibody [EPR23696-22] (ab230822)
    Immunoprecipitation - Anti-BMAL1 antibody [EPR23696-22] (ab230822)

    ab230822 at 1/30 (2μg in 0.35mg lysates) immunoprecipitating BMAL1 in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate.

    Lane 1 (input): NIH/3T3 whole cell lysate (10µg)

    Lane 2 (+): ab230822 + NIH/3T3 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab230822 in NIH/3T3 whole cell lysate.

    For western blotting, ab230822 at 1/1000 dilution (0.58 μg/mL) and ab131366 VeriBlot for IP (HRP) at 1/5000 was used for detection.

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    Fresh lysates were used in this IP.

  • Western blot - Anti-BMAL1 antibody [EPR23696-22] (ab230822)
    Western blot - Anti-BMAL1 antibody [EPR23696-22] (ab230822)
    Anti-BMAL1 antibody [EPR23696-22] (ab230822) at 1/1000 dilution + PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate 20 at 20 µg

    Secondary
    VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 68 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST The molecular weight observed is consistent with what has been described in the literature (PMID: 28332504).  Exposure time: 70 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMAL1 antibody [EPR23696-22] (ab230822)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMAL1 antibody [EPR23696-22] (ab230822)

    Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling BMAL1 with ab230822 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in mouse hippocampus (PMID: 20382135). The section was incubated with ab230822 for 20 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Antigen retrieval was heat mediated with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

  • Immunoprecipitation - Anti-BMAL1 antibody [EPR23696-22] (ab230822)
    Immunoprecipitation - Anti-BMAL1 antibody [EPR23696-22] (ab230822)

    ab230822 at 1/30 (2μg in 0.35mg lysates) immunoprecipitating BMAL1 in PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate.

    Lane 1 (input): PC-3 whole cell lysate (10µg)

    Lane 2 (+): ab230822 + PC-3 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab230822 in PC-3 whole cell lysate.

    For western blotting, ab230822 at 1/1000 dilution (0.58 μg/mL) and ab131366 VeriBlot for IP (HRP) at 1/5000 was used for detection.

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    Fresh lysates were used in this IP.
    The molecular weight observed is consistent with what has been described in the literature (PMID: 28332504).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMAL1 antibody [EPR23696-22] (ab230822)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMAL1 antibody [EPR23696-22] (ab230822)

    Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling BMAL1 with ab230822 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in mouse cardiac muscle (PMID: 10760301). The section was incubated with ab230822 for 20 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Antigen retrieval was heat mediated with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

  • Anti-BMAL1 antibody [EPR23696-22] (ab230822)
    Anti-BMAL1 antibody [EPR23696-22] (ab230822)

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