Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µg 284 784 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Mouse monoclonal [7A3BB6] to BAP31
Suitable for: IHC-P, ICC/IF, WB, Flow Cyt
Knockout validated
Reacts with: Mouse, Human
Isotype: IgG1

Product name

Anti-BAP31 antibody [7A3BB6]
See all BAP31 primary antibodies
Description

Mouse monoclonal [7A3BB6] to BAP31
Host species

Mouse
Specificity

Shotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Tissue, cells or virus. This information is considered to be commercially sensitive.
Positive control
- WB: HEK293T, HeLa, NCCIT and HepG2 cell lysates. Flow Cyt: HL60 cells. ICC/IF: Human fibroblast cells. IHC-P: Human normal colon FFPE tissue.
General notes

This antibody clone is manufactured by Abcam.

Product was previously marketed under the MitoSciences sub-brand.

If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

Preservative: 0.02% Sodium azide
Constituent: 99.98% HEPES buffered saline
Concentration information loading...
Purity

Proprietary Purification
Purification notes

ab112993 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE (>95%).
Clonality

Monoclonal
Clone number

7A3BB6
Isotype

IgG1
Light chain type

kappa
Research areas

Western blot - Anti-BAP31 antibody [7A3BB6] (ab112993)

All lanes : Anti-BAP31 antibody [7A3BB6] (ab112993) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : BCAP31 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : NCCIT cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution

Predicted band size: 28 kDa
Observed band size: 28 kDa

Lanes 1-4: Merged signal (red and green). Green - ab112993 observed at 28 kDa. Red - loading control ab52901 observed at kDa.

ab112993 Anti-BAP31 antibody [7A3BB6] was shown to specifically react with BAP31 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266634 (knockout cell lysate ab257857) was used. Wild-type and BAP31 knockout samples were subjected to SDS-PAGE. ab112993 and Anti-beta Tubulin [EP1331Y] - Microtubule Marker (ab52901) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Flow Cytometry - Anti-BAP31 antibody [7A3BB6] (ab112993)

ab112993 at 1ug/ml staining BAP31 in MeOH-fixed HL60 cells by Flow Cytometry (blue). Isotype control antibody (red).
Immunocytochemistry/ Immunofluorescence - Anti-BAP31 antibody [7A3BB6] (ab112993)

ab112993 at 5 µg/ml staining BAP31 in Human fibroblast cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor^® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (green). The target locates mainly in the endoplasmic reticulum.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody [7A3BB6] (ab112993)

IHC image of BAP31 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112993, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-BAP31 antibody [7A3BB6] (ab112993)

All lanes : Anti-BAP31 antibody [7A3BB6] (ab112993) at 0.5 µg/ml

Lane 1 : HepG2 Cell Lysate
Lane 2 : H9C2 Cell Lysate
Lane 3 : MEF Cell Lysate

Predicted band size: 28 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com