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Anti-BAP31 antibody [7A3BB6] (ab112993)

Key features and details

  • Mouse monoclonal [7A3BB6] to BAP31
  • Suitable for: IHC-P, ICC/IF, WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-BAP31 antibody [7A3BB6]
    See all BAP31 primary antibodies

  • Description

    Mouse monoclonal [7A3BB6] to BAP31

  • Host species

    Mouse

  • Specificity

    Shotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • WB: HEK293T, HeLa, NCCIT and HepG2 cell lysates. Flow Cyt: HL60 cells. ICC/IF: Human fibroblast cells. IHC-P: Human normal colon FFPE tissue.

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99.98% HEPES buffered saline
  • Concentration information loading...

  • Purity

    Proprietary Purification

  • Purification notes

    ab112993 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE (>95%).

  • Clonality

    Monoclonal

  • Clone number

    7A3BB6

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Images

  • Western blot - Anti-BAP31 antibody [7A3BB6] (ab112993)
    Western blot - Anti-BAP31 antibody [7A3BB6] (ab112993)
    All lanes : Anti-BAP31 antibody [7A3BB6] (ab112993) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : BCAP31 knockout HEK293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : NCCIT cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab112993 observed at 28 kDa. Red - loading control ab52901 observed at  kDa.

     ab112993 Anti-BAP31 antibody [7A3BB6] was shown to specifically react with BAP31 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266634 (knockout cell lysate ab257857) was used. Wild-type and BAP31 knockout samples were subjected to SDS-PAGE. ab112993 and Anti-beta Tubulin [EP1331Y] - Microtubule Marker (ab52901) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-BAP31 antibody [7A3BB6] (ab112993)
    Flow Cytometry - Anti-BAP31 antibody [7A3BB6] (ab112993)
    ab112993 at 1ug/ml staining BAP31 in MeOH-fixed HL60 cells by Flow Cytometry (blue). Isotype control antibody (red).
  • Immunocytochemistry/ Immunofluorescence - Anti-BAP31 antibody [7A3BB6] (ab112993)
    Immunocytochemistry/ Immunofluorescence - Anti-BAP31 antibody [7A3BB6] (ab112993)

    ab112993 at 5 µg/ml staining BAP31 in Human fibroblast cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (green). The target locates mainly in the endoplasmic reticulum.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody [7A3BB6] (ab112993)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody [7A3BB6] (ab112993)

    IHC image of BAP31 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112993, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-BAP31 antibody [7A3BB6] (ab112993)
    Western blot - Anti-BAP31 antibody [7A3BB6] (ab112993)
    All lanes : Anti-BAP31 antibody [7A3BB6] (ab112993) at 0.5 µg/ml

    Lane 1 : HepG2 Cell Lysate
    Lane 2 : H9C2 Cell Lysate
    Lane 3 : MEF Cell Lysate

    Predicted band size: 28 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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