Anti-Kinetochore antibody (ab85996)
Key features and details
- Rabbit polyclonal to Kinetochore
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Kinetochore antibody
See all Kinetochore primary antibodies -
Description
Rabbit polyclonal to Kinetochore -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to a region within residues 375 and 425 of human Kinetochore (NP_055523.1).
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Positive control
- HeLa whole cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab85996 was affinity purified using an epitope specific to Kinetochore immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Kinetochore antibody (ab85996)All lanes : Anti-Kinetochore antibody (ab85996) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 250 kDa
Observed band size: 250 kDa
Additional bands at: 115 kDa, 175 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds
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Immunoprecipitation - Anti-Kinetochore antibody (ab85996)Detection of Kinetochore by Western Blot of Immunprecipitate.
ab85996, at 1 µg/ml, staining Kinetochore in HeLa whole cell lysate immunoprecipitated using ab8599 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
