DNase I Assay Kit (Fluorometric) (ab234056)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Purified protein, Serum
Overview
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Product name
DNase I Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Serum, Purified protein -
Assay type
Enzyme activity (quantitative) -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
DNase I Assay Kit (Fluorometric) (ab234056) allows for quantitative evaluation of DNase I activity of purified enzymes and their inhibitors as well as comparative examination of DNase I activity in biological samples. Enzyme activity is detected upon cleavage of a DNA Probe, which yields a fluorescent DNA product measured at Ex/Em = 651/681 nm. The limit of quantification (L.O.Q) is 178 fmoles of DNA probe cleaved per minute per mL.
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Notes
Deoxyribonuclease I (DNase I) is an endonuclease that cleaves DNA phosphodiester bonds yielding 5′-phosphorylated and 3′-hydroxylated oligonucleotides. DNase I targets single-stranded DNA, double-stranded DNA, and chromatin in a non-specific manner. As an important player in cellular waste management, DNase I is normally secreted extracellularly to clear the system from circulating cell-free DNA, foreign DNA from food digestion or potential pathogens, and endogenous chromosomal DNA from apoptotic and necrotic cells. Abnormal DNase I activity occurs in association with a variety of cancers and auto-immune illnesses that exhibit elevated levels of cell-free DNA. Furthermore, DNase I has been therapeutically used in cystic fibrosis patients to degrade DNA and reduce sputum viscosity.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 10X DNase I Assay Buffer 1 x 1.1ml DNA Probe 1 vial DNA Probe Re-suspension Buffer 1 x 250µl DNAse I Positive Control 1 vial Molecular Biology Grade Water 1 x 25ml Positive Control Re-suspension Buffer 1 x 1ml -
Research areas
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Function
Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. -
Tissue specificity
Principally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva. -
Sequence similarities
Belongs to the DNase I family. -
Cellular localization
Secreted. Nucleus envelope. Secretory protein, stored in zymogen granules and found in the nuclear envelope. - Information by UniProt
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Alternative names
- Deoxyribonuclease 1
- Deoxyribonuclease I
- Deoxyribonuclease-1
see all
Images
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DNA Probe to Product conversion standard curve.
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Representative activity curve for purified DNAse I (orange), serum sample (green), and background control (blue) at 37°C. -
Comparative analysis of DNAse I activity from 25 µl undiluted single donor normal vs. Systematic Lupus Erythematosus (SLE) patient serum sample.