Anti-Bub1 antibody (ab227228)
Key features and details
- Rabbit polyclonal to Bub1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Bub1 antibody
See all Bub1 primary antibodies -
Description
Rabbit polyclonal to Bub1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment within Human Bub1 (N terminal). The exact sequence is proprietary.
Database link: O43683 -
Positive control
- WB: A431 and HEK-293T whole cell lysates. ICC/IF: U-2 OS cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Bub1 antibody (ab227228)Anti-Bub1 antibody (ab227228) at 1/3000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 122 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-Bub1 antibody (ab227228)Anti-Bub1 antibody (ab227228) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Predicted band size: 122 kDa5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-Bub1 antibody (ab227228)Paraformaldehyde-fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Bub1 (green) using ab227228 at 1/500 dilution in ICC/IF. Counterstain: Alpha Tubulin filaments were labeled with an anti-alpha Tubulin antibody (red).
