Phospholipase C Activity Assay Kit (Colorimetric) (ab273343)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Tissue
Overview
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Product name
Phospholipase C Activity Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Tissue -
Assay type
Enzyme activity (quantitative) -
Assay duration
Multiple steps standard assay -
Product overview
Phospholipase C Activity Assay Kit (Colorimetric) (ab273343) is a direct assay to determine the Phospholipase C (PLC) activity in various biological samples. The assay uses a specific PLC chromogenic substrate to detect PLC activity and the generated final product can be measured colorimetrically at OD 405 nm. The OD signal is proportional to the PLC activity.
The assay is rapid, sensitive and is a convenient tool for detecting PLC activity.
It can detect as low as 0.1 mU PLC activity under the assay conditions.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests PLC Assay Buffer 1 x 25ml PLC Enzyme Lyophilized 1 vial PLC Standard 1 x 100µl PLC Substrate 1 vial
Images
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Example data
Example data.
PLC Standard Curve.
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Example dataExample data.
Reaction curve of the PLC activity.
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Example dataExample data.
PLC activity detected in rat brain, kidney and muscle lysates.