Recombinant mouse BAFF protein (ab157285)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20ºC.
Constituent: 99% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with 100µl sterile water to a concentration of 0.1 mg/ml. Further dilutions should be made with medium containing 5% fetal calf serum or other carrier protein. After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Images
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SDS-PAGE - Recombinant mouse BAFF protein (ab157285)SDS-PAGE analysis: Lane 1: MWt Marker, Lane 2: 1 μg ab157285, stained with Imperial stain
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Functional Studies - Recombinant mouse BAFF protein (ab157285)ab157285 mediates survival of freshly isolated splenocytes.Method: On day 0 splenocytes were isolated from a freshly collected C57Bl6 spleen. An aliquot of the splenocytes was analyzed on FACS and gated on the SSC-FSC panel. FACS settings were saved. The rest of the cells was put in culture with media alone or with increasing concentrations of ab157285 as indicated. After three days in culture, cells were harvested and analyzed on FACS with the saved setting. Splenocytes Survival Index (ratio % living/% dead cells) was calculated and plotted.
