Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BAP31 antibody [EPR3878(2)] (ab109304)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3878(2)] to BAP31
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-BAP31 antibody [EPR3878(2)]
    See all BAP31 primary antibodies

  • Description

    Rabbit monoclonal [EPR3878(2)] to BAP31

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T, NCCIT and HeLa cell lysates; Human placenta tissue lysate. IHC-P: Human kidney tissue and Human brain tissue. ICC/IF: HeLa cells.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR3878(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-BAP31 antibody [EPR3878(2)] (ab109304)
    Western blot - Anti-BAP31 antibody [EPR3878(2)] (ab109304)
    All lanes : Anti-BAP31 antibody [EPR3878(2)] (ab109304) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : BCAP31 knockout HEK293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : NCCIT cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab109304 observed at 28 kDa. Red - loading control ab7291 observed at 50 kDa.

     ab109304 Anti-BAP31 antibody [EPR3878(2)] was shown to specifically react with BAP31 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266634 (knockout cell lysate ab257857) was used. Wild-type and BAP31 knockout samples were subjected to SDS-PAGE. ab109304 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody [EPR3878(2)] (ab109304)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody [EPR3878(2)] (ab109304)

    ab109304, at 1/250 dilution, staining Human kidney tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-BAP31 antibody [EPR3878(2)] (ab109304)
    Immunocytochemistry/ Immunofluorescence - Anti-BAP31 antibody [EPR3878(2)] (ab109304)
    ab109304, at 1/100 dilution, staining HeLa cells by immunofluorescence.
  • Western blot - Anti-BAP31 antibody [EPR3878(2)] (ab109304)
    Western blot - Anti-BAP31 antibody [EPR3878(2)] (ab109304)
    All lanes : Anti-BAP31 antibody [EPR3878(2)] (ab109304) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Human placenta tissue lysate
    Lane 3 : NCCIT cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 28 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody [EPR3878(2)] (ab109304)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody [EPR3878(2)] (ab109304)

    ab109304, at 1/250 dilution, staining Human brain tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-BAP31 antibody [EPR3878(2)] (ab109304)
    Anti-BAP31 antibody [EPR3878(2)] (ab109304)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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