Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-BAP31 antibody [EPR3878(2)] (ab109304)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3878(2)] to BAP31
Suitable for: WB, IHC-P, ICC/IF
Knockout validated
Reacts with: Human

Product name

Anti-BAP31 antibody [EPR3878(2)]
See all BAP31 primary antibodies
Description

Rabbit monoclonal [EPR3878(2)] to BAP31
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK293T, NCCIT and HeLa cell lysates; Human placenta tissue lysate. IHC-P: Human kidney tissue and Human brain tissue. ICC/IF: HeLa cells.
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3878(2)
Isotype

IgG
Research areas

Western blot - Anti-BAP31 antibody [EPR3878(2)] (ab109304)

All lanes : Anti-BAP31 antibody [EPR3878(2)] (ab109304) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : BCAP31 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : NCCIT cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 28 kDa
Observed band size: 28 kDa

Lanes 1-4: Merged signal (red and green). Green - ab109304 observed at 28 kDa. Red - loading control ab7291 observed at 50 kDa.

ab109304 Anti-BAP31 antibody [EPR3878(2)] was shown to specifically react with BAP31 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266634 (knockout cell lysate ab257857) was used. Wild-type and BAP31 knockout samples were subjected to SDS-PAGE. ab109304 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody [EPR3878(2)] (ab109304)

ab109304, at 1/250 dilution, staining Human kidney tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-BAP31 antibody [EPR3878(2)] (ab109304)

ab109304, at 1/100 dilution, staining HeLa cells by immunofluorescence.
Western blot - Anti-BAP31 antibody [EPR3878(2)] (ab109304)

All lanes : Anti-BAP31 antibody [EPR3878(2)] (ab109304) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Human placenta tissue lysate
Lane 3 : NCCIT cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 28 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody [EPR3878(2)] (ab109304)

ab109304, at 1/250 dilution, staining Human brain tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-BAP31 antibody [EPR3878(2)] (ab109304)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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