Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP356(2)AY] to p53 (acetyl K373)
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-p53 (acetyl K373) antibody [EP356(2)AY]
    See all p53 primary antibodies

  • Description

    Rabbit monoclonal [EP356(2)AY] to p53 (acetyl K373)

  • Host species

    Rabbit

  • Specificity

    ab62376 only detects p53 acetylated on Lysine 373.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. within Human p53 aa 350 to the C-terminus (acetyl K373). The exact sequence is proprietary.
    Database link: P04637
    (Peptide available as ab186195)

  • Positive control

    • ICC/IF: HepG2 cell lysates treated with etoposide + trichostatin A; IHC-P: Human cervical carcinoma; WB: A431 and HepG2 cell lysates treated with etoposide + trichostatin A; Flow Cyt: HeLa (human epithelial cell line from cervix adenocarcinoma) cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376)
    Western blot - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376)
    All lanes : Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376) at 1/5000 dilution

    Lane 1 : Untreated A431 whole cell lysate
    Lane 2 : A431 treated with 30ug/mL etoposide for 8 hours, then treated with 500 ng/mL trichostatin A for 4hours, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    Blocking and Diluting buffer 5% NFDM/TBST
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376)

    Immunohistochemical analysis of paraffin-embedded human cervical carcinoma sections labelling p53 (acetyl K373) with purified ab62376 at dilution of 1/800. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376)
    Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376)

    Immunocytochemistry/Immunofluorescence analysis of HepG2 +/- (etoposide + trichostatin A) (etoposide 30ug/ml 8hr followed by TSA 500ng/ml 4hr) cells labelling p53 (acetyl K373) with purified ab62376 at 1/250. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291 anti-Tubulin (mouse mAb) primary and ab150120 (AlexaFluor®594 goat anti-mouse) secondary both at 1/1000 dilution. Nuclei were counterstained with DAPI (blue).

    For negative control 1, rabbit primary antibody and ab150120 (anti-mouse) secondary antibody were used. For negative control 2, ab7291 (mouse primary antibody) was used followed by ab150077 (anti-rabbit secondary antibody).

  • Flow Cytometry - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376)
    Flow Cytometry - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376)

    Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling p53 (acetyl K373) with purified ab62376 at 1/120 (red). Cells were fixed with 4% paraformaldehyde and then permeabilized with 90% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] (ab172730). Unlabelled sample (blue line) was also used as a control.

  • Western blot - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376)
    Western blot - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376)
    All lanes : Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376) at 1/5000 dilution

    Lane 1 : Untreated HepG2 whole cell lysate
    Lane 2 : HepG2 treated with 30 ug/mL etoposide for 8 hours,then treated with 500 ng/mL trichostatin A for 4hours, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    Blocking and Diluting buffer 5% NFDM/TBST
  • Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376)
    Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376)

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