Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 02, 2021

Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP356(2)AY] to p53 (acetyl K373)
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-p53 (acetyl K373) antibody [EP356(2)AY]
See all p53 primary antibodies
Description

Rabbit monoclonal [EP356(2)AY] to p53 (acetyl K373)
Host species

Rabbit
Specificity

ab62376 only detects p53 acetylated on Lysine 373.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. within Human p53 aa 350 to the C-terminus (acetyl K373). The exact sequence is proprietary.
Database link: P04637
(Peptide available as ab186195)
Positive control
- ICC/IF: HepG2 cell lysates treated with etoposide + trichostatin A; IHC-P: Human cervical carcinoma; WB: A431 and HepG2 cell lysates treated with etoposide + trichostatin A; Flow Cyt: HeLa (human epithelial cell line from cervix adenocarcinoma) cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.11% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP356(2)AY
Isotype

IgG
Research areas

Western blot - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376)

All lanes : Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376) at 1/5000 dilution

Lane 1 : Untreated A431 whole cell lysate
Lane 2 : A431 treated with 30ug/mL etoposide for 8 hours, then treated with 500 ng/mL trichostatin A for 4hours, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 53 kDa
Observed band size: 53 kDa

Blocking and Diluting buffer 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376)

Immunohistochemical analysis of paraffin-embedded human cervical carcinoma sections labelling p53 (acetyl K373) with purified ab62376 at dilution of 1/800. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376)

Immunocytochemistry/Immunofluorescence analysis of HepG2 +/- (etoposide + trichostatin A) (etoposide 30ug/ml 8hr followed by TSA 500ng/ml 4hr) cells labelling p53 (acetyl K373) with purified ab62376 at 1/250. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291 anti-Tubulin (mouse mAb) primary and ab150120 (AlexaFluor^®594 goat anti-mouse) secondary both at 1/1000 dilution. Nuclei were counterstained with DAPI (blue).

For negative control 1, rabbit primary antibody and ab150120 (anti-mouse) secondary antibody were used. For negative control 2, ab7291 (mouse primary antibody) was used followed by ab150077 (anti-rabbit secondary antibody).
Flow Cytometry - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376)

Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling p53 (acetyl K373) with purified ab62376 at 1/120 (red). Cells were fixed with 4% paraformaldehyde and then permeabilized with 90% methanol. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] (ab172730). Unlabelled sample (blue line) was also used as a control.
Western blot - Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376)

All lanes : Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376) at 1/5000 dilution

Lane 1 : Untreated HepG2 whole cell lysate
Lane 2 : HepG2 treated with 30 ug/mL etoposide for 8 hours,then treated with 500 ng/mL trichostatin A for 4hours, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 53 kDa
Observed band size: 53 kDa

Blocking and Diluting buffer 5% NFDM/TBST
Anti-p53 (acetyl K373) antibody [EP356(2)AY] (ab62376)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com