Anti-PAK1 + PAK2 + PAK3 (phospho T402) antibody (ab30577)
Key features and details
- Rabbit polyclonal to PAK1 + PAK2 + PAK3 (phospho T402)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-PAK1 + PAK2 + PAK3 (phospho T402) antibody
See all PAK1 + PAK2 + PAK3 primary antibodies -
Description
Rabbit polyclonal to PAK1 + PAK2 + PAK3 (phospho T402) -
Host species
Rabbit -
Specificity
ab30577 recognises PAK1 + PAK2 + PAK3 (phospho T402). -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat
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Immunogen
Synthetic Phosphopeptide corresponding to amino acid residues surrounding the phospho Thr402 of rat p21 Activated Kinase 2 (PAK2). The peptide sequence used is identical in PAK1, 2 and 3.
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Positive control
- Rat hippocampal lysate
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General notes
Note: Thr402 in PAK2 corresponds to Thr423 in human PAK1.Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab30577 was purified by sequential chromatography on phospho and dephosphopeptide affinity columns. -
Primary antibody notes
Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PAK1 + PAK2 + PAK3 (phospho T402) antibody (ab30577)All lanes : Anti-PAK1 + PAK2 + PAK3 (phospho T402) antibody (ab30577) at 1/1000 dilution
Lane 1 : untreated rat hippocampal lysate
Lane 2 : rat hippocampal lysate, pretreated with 1200 units lambda Ptase for 30 min (negative control)
Predicted band size: 60 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
