Anti-PAK1 + PAK2 + PAK3 (phospho T423) antibody (ab2477)
Key features and details
- Rabbit polyclonal to PAK1 + PAK2 + PAK3 (phospho T423)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PAK1 + PAK2 + PAK3 (phospho T423) antibody
See all PAK1 + PAK2 + PAK3 primary antibodies -
Description
Rabbit polyclonal to PAK1 + PAK2 + PAK3 (phospho T423) -
Host species
Rabbit -
Specificity
This phospho specific polyclonal antibody is specific for phosphorylated Threonine 423 of Human PAK 1 + 2 + 3. Reactivity with non-phosphorylated PAK 1 + 2 + 3 is less than 1% by ELISA. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide: EQSKRSTpMVGTPY conjugated to KLH, corresponding to amino acids 413-433 of Human PAK 1, 408-428 of human PAK 2 and 411-431 of human PAK 3.
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General notes
P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of threonine 423 (threonine 402 for PAK 2 and threonine 421 for PAK 3), catalyzed by Cdc42, is required for activation of PAK.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. -
Primary antibody notes
P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of threonine 423 (threonine 402 for PAK 2 and threonine 421 for PAK 3), catalyzed by Cdc42, is required for activation of PAK. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PAK1 + PAK2 + PAK3 (phospho T423) antibody (ab2477)
Western blot probing with PAK1 antibody (ab2477). The major band is at 65 kDa and weaker bands can be detected at 90 and 100 kDa. This picture was kindly submitted by Sue Thomas as part of her review.
Western blot probing with PAK1 antibody (ab2477). The major band is at 65 kDa and weaker bands can be detected at 90 and 100 kDa. This picture was kindly submitted by Sue Thomas as part of her review.
