Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8011] to Cathepsin L/V/K/H
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, ELISA
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-Cathepsin L/V/K/H antibody [EPR8011]
    See all Cathepsin L/V/K/H primary antibodies

  • Description

    Rabbit monoclonal [EPR8011] to Cathepsin L/V/K/H

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Cathepsin L/V/K/H aa 100-200. The exact sequence is proprietary.
    Database link: P07711

  • Positive control

    • WB: A549, HCT-116, NIH/3T3, PC-12 and HepG2 whole cell lysate (ab7900). IHC-P: Human kidney tissue. ICC/IF: HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)

    Antigen: CATL_HUMAN, CATV_HUMAN, CATS_HUMAN, CATK_HUMAN, CATH_HUMAN

    Antigen concentration: 1000ng/ml

    Primary antibody concentration range: 0~1000ng/ml

    Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Secondary antibody concentration: 1/2500

  • Flow Cytometry - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Flow Cytometry - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)

    ab133641 staining Cathepsin L in the human cell line HepG2 (human hepatocellular carcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Lane 1 : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/20 dilution
    Lane 2 : Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/100 dilution
    Lane 3 : Anti-His tag at 1/10000 dilution

    All lanes : Human Cathepsin L recombinant protein fraction

    Secondary
    Lanes 1 & 3 : Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/1000 dilution
    Lane 2 : Rabbit anti-mouse IgG at 1/2000 dilution

    Predicted band size: 38 kDa



    Blocking buffer and concentration:5% NFDM/TBST

    Diluting buffer and concentration:5% NFDM/TBST

    Observed MW:30

    Exposure time:3 minutes

  • Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Lane 1 : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/20 dilution
    Lane 2 : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/100 dilution
    Lane 3 : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/500 dilution
    Lane 4 : Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/100 dilution
    Lane 5 : Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/1000 dilution
    Lane 6 : Anti-His tag at 1/10000 dilution

    All lanes : Human Cathepsin V recombinant protein fraction

    Secondary
    Lanes 1-3 : Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/1000 dilution
    Lanes 4-5 : Rabbit Anti-Mouse secondary ab at 1/2000 dilution
    Lane 6 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa



    Blocking buffer and concentration:5% NFDM/TBST

    Diluting buffer and concentration:5% NFDM/TBST

    Observed MW:30

    Exposure time:3 minutes

  • ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)

    Direct ELISA antigen dose-response curve using purified ab133641.Antigen concentration of 1000ng/mL.Alkaline Phosphatase conjugated AffiniPure goat anti-rabbit IgG(H+L)(1/2500) was used as the secondary antibody.

  • Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/20000 dilution (purified) + HepG2 cell lysate at 20 µg

    Secondary
    Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    All lanes : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/10000 dilution (purified)

    Lane 1 : HCT-116 cell lysate
    Lane 2 : A549 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    All lanes : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/20000 dilution (purified)

    Lane 1 : NIH/3T3 cell lysate
    Lane 2 : PC-12 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    All lanes : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/1000 dilution (unpurified)

    Lane 1 : A549 cell lysate
    Lane 2 : HCT-116 cell lysate
    Lane 3 : NIH/3T3 cell lysate
    Lane 4 : PC-12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 38 kDa

  • Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/1000 dilution (unpurified) + HepG2 cell lysate at 10 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 38 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Cathepsin L with purified ab133641 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Cathepsin L with unpurified ab133641 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)

    Immunocytochemistry/Immunofluorescence analysis of HT-29 cells labelling Cathepsin L with purified ab133641 at 1/100. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) This image is courtesy of an Abreview submitted by Alina Macovei

    Unpurified ab133641 staining Cathepsin L in Human HepaRG cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% milk for 30 minutes at room temperature. Samples were incubated with primary antibody (1/1000) for 30 minutes. An Alexa Fluor® 488-conjugated Donkey anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.

    See Abreview

  • OI-RD Scanning - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    OI-RD Scanning - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)

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