Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PMP22 antibody [CF1] (ab90782)

Key features and details

  • Mouse monoclonal [CF1] to PMP22
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-PMP22 antibody [CF1]
    See all PMP22 primary antibodies

  • Description

    Mouse monoclonal [CF1] to PMP22

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse

  • Immunogen

    Synthetic peptide:

    CRHPEWHLNSDYSYG

    corresponding to amino acids 121-133 from the second extracellular domain of Human PMP22 with an N-terminal Cysteine residue linker
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human nerve section

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Anti-PMP22 antibody [CF1] (ab90782)
    Flow Cytometry - Anti-PMP22 antibody [CF1] (ab90782)
    Overlay histogram showing SH-SY5Y cells stained with ab90782 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab90782, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [CF1] (ab90782)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [CF1] (ab90782)
    Formalin fixed, paraffin embedded human nerve tissue labelled with ab90782 at 1 µg/ml. Positive staining of peripheral nerve myelin sheaths can be observed. Image = 20x magnification.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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