Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PMP22 antibody [EPR23112-110] - BSA and Azide free (ab270407)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23112-110] to PMP22 - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-PMP22 antibody [EPR23112-110] - BSA and Azide free
    See all PMP22 primary antibodies

  • Description

    Rabbit monoclonal [EPR23112-110] to PMP22 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human nerve tissue. ICC/IF: 293T transfected with myc-his tagged PMP22 overexpression construct cells. Flow Cyt: 293T transfected with myc-his tagged PMP22 overexpression construct cells. IP: 293T transfected with myc-his tagged PMP22 overexpression construct cells.

  • General notes

    ab270407 is the carrier-free version of ab270400.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-PMP22 antibody [EPR23112-110] - BSA and Azide free (ab270407)
    Immunoprecipitation - Anti-PMP22 antibody [EPR23112-110] - BSA and Azide free (ab270407)

    PMP22  was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) transfected with PMP22 (WT) expression vector containing a myc-His-tag®, whole cell lysate with ab270400 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270400 1/1000 dilution (0.58 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: 293T cells transfected with PMP22 (WT) expression vector containing a myc-His-tag®, whole cell lysate 10 μg

    Lane 2: ab270400 IP in 293T transfected with PMP22 (WT) expression vector containing a myc-His-tag®, whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270400 in 293T transfected with PMP22 (WT) expression vector containing a myc-His-tag®, whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270400).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR23112-110] - BSA and Azide free (ab270407)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR23112-110] - BSA and Azide free (ab270407)

    Immunohistochemical analysis of paraffin-embedded Human nerve tissue labeling PMP22 with ab270400 at 1/32000 dilution (0.018 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in Schwann cells of human nerve (PMID:7691737) is observed. The section was incubated with ab270400 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270400).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR23112-110] - BSA and Azide free (ab270407)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP22 antibody [EPR23112-110] - BSA and Azide free (ab270407)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PMP22  with ab270400 at 1/32000 dilution (0.018 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Negative control: negative staining in human kidney (PMID:7649472).

    The section was incubated with ab270400 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270400).

  • Immunocytochemistry/ Immunofluorescence - Anti-PMP22 antibody [EPR23112-110] - BSA and Azide free (ab270407)
    Immunocytochemistry/ Immunofluorescence - Anti-PMP22 antibody [EPR23112-110] - BSA and Azide free (ab270407)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells transfected with a myc-tagged PMP22 expression construct. cells labelling PMP22 with ab270400 at 1/250 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in 293T cells transfected with a myc-tagged PMP22 expression construct is observed. 2233S Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270400).

  • Flow Cytometry - Anti-PMP22 antibody [EPR23112-110] - BSA and Azide free (ab270407)
    Flow Cytometry - Anti-PMP22 antibody [EPR23112-110] - BSA and Azide free (ab270407)

    Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized 293T transfected with myc-his tagged PMP22 overexpression construct cells labelling PMP22 with ab270400 at 1/600 dilution (0.1 ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with isotype control or ab270400. Then fixed with 2% PFA for 10min followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor® 647.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270400).

  • Anti-PMP22 antibody [EPR23112-110] - BSA and Azide free (ab270407)
    Anti-PMP22 antibody [EPR23112-110] - BSA and Azide free (ab270407)

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