Anti-OP-2 antibody [EPR13357] - BSA and Azide free (ab250466)
![Anti-OP-2 antibody [EPR13357] - BSA and Azide free (ab250466)](https://www.abcam.com/ps/products/250/ab250466/Images/ab250466-402755-anti-op-2-antibody-epr13357-bsa-and-azide-free-western-blot.jpg "Anti-OP-2 antibody [EPR13357] - BSA and Azide free (ab250466)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13357] to OP-2 - BSA and Azide free
- Suitable for: ICC, WB
- Reacts with: Human
Overview
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Product name
Anti-OP-2 antibody [EPR13357] - BSA and Azide free
See all OP-2 primary antibodies -
Description
Rabbit monoclonal [EPR13357] to OP-2 - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody detects dominant ~35kDa extra band in some samples, e.g. WI-38 and SK-OV-3.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250466 is the carrier-free version of ab181253. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250466 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13357 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-OP-2 antibody [EPR13357] - BSA and Azide free (ab250466)All lanes : Anti-OP-2 antibody [EPR13357] (ab181253) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 45 kDaThis data was developed using ab181253, the same antibody clone in a different buffer formulation.
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![Immunocytochemistry - Anti-OP-2 antibody [EPR13357] - BSA and Azide free (ab250466)](https://www.abcam.com/ps/products/250/ab250466/Images/ab250466-1-anti-op-2-antibody-epr13357-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-OP-2 antibody [EPR13357] - BSA and Azide free (ab250466)This data was developed using ab181253, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of, paraformaldehyde-fixed, A549 cells labeling OP-2 with ab181253 at a 1/250 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor®488) was used at a 1/200 dilution. In blue DAPI staining. -
![Anti-OP-2 antibody [EPR13357] - BSA and Azide free (ab250466)](https://www.abcam.com/ps/products/250/ab250466/Images/ab250466-3-benefits-of-recombinant-antibodies.png)
Anti-OP-2 antibody [EPR13357] - BSA and Azide free (ab250466)
