Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-POR1 antibody [EPR11943] - BSA and Azide free (ab250206)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11943] to POR1 - BSA and Azide free
  • Suitable for: IHC-P, ICC, IP, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-POR1 antibody [EPR11943] - BSA and Azide free
    See all POR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11943] to POR1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC, IP, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250206 is the carrier-free version of ab180503. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250206 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR11943

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-POR1 antibody [EPR11943] - BSA and Azide free (ab250206)
    Western blot - Anti-POR1 antibody [EPR11943] - BSA and Azide free (ab250206)
    All lanes : Anti-POR1 antibody [EPR11943] (ab180503) at 1/5000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa



    This data was developed using ab180503, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-POR1 antibody [EPR11943] - BSA and Azide free (ab250206)
    Immunoprecipitation - Anti-POR1 antibody [EPR11943] - BSA and Azide free (ab250206)
    This data was developed using ab180503, the same antibody clone in a different buffer formulation.Western blot analysis of SH-SY5Y cell lysate (Lane 1) and Negative control (Lane 2) immunoprecipitated with ab180503 at 1/30 dilution. Rabbit TrueBlot®:Anti-Rabbit IgG HRP secondary antibody used at 1/1500 dilution.
  • Flow Cytometry - Anti-POR1 antibody [EPR11943] - BSA and Azide free (ab250206)
    Flow Cytometry - Anti-POR1 antibody [EPR11943] - BSA and Azide free (ab250206)
    This data was developed using ab180503, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed SH-SY5Y cells labeling POR2 with ab180503 at 1/150 dilution (red), compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/75 dilution.
  • Immunocytochemistry - Anti-POR1 antibody [EPR11943] - BSA and Azide free (ab250206)
    Immunocytochemistry - Anti-POR1 antibody [EPR11943] - BSA and Azide free (ab250206)
    This data was developed using ab180503, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed SH-SY5Y cells labeling POR2 with ab180503 at 1/1000 dilution, followed by Goat anti rabbit IgG (Dylight 555) secondary antibody at 1/250 dilution. Counter stained with DAPI.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POR1 antibody [EPR11943] - BSA and Azide free (ab250206)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POR1 antibody [EPR11943] - BSA and Azide free (ab250206)
    This data was developed using ab180503, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling POR2 with ab180503 at 1/500 dilution, followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Anti-POR1 antibody [EPR11943] - BSA and Azide free (ab250206)
    Anti-POR1 antibody [EPR11943] - BSA and Azide free (ab250206)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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