Anti-p27 KIP 1 antibody (ab215434)
Key features and details
- Rabbit polyclonal to p27 KIP 1
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-p27 KIP 1 antibody
See all p27 KIP 1 primary antibodies -
Description
Rabbit polyclonal to p27 KIP 1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human p27 KIP 1 aa 150 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P46527 -
Positive control
- Human ovary carcinoma tissue; MCF-7 cells; rat RSC96 cells; mouse lung lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-p27 KIP 1 antibody (ab215434)Anti-p27 KIP 1 antibody (ab215434) at 1/300 dilution (overnight at 4°C) + Mouse lung lysates
Secondary
conjugated secondary antibody (60 minutes at 37°C) at 1/10000 dilution
Predicted band size: 22 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody (ab215434)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human ovary carcinoma tissue labeling p27 KIP 1 with ab215434 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Flow Cytometry - Anti-p27 KIP 1 antibody (ab215434)Flow cytometric analysis of rat RSC96 cells labeling p27 KIP 1 with ab215434 at 1/100 dilution for 30 minutes, followed by incubation with a PE conjugated secondary for 30 minutes (green), compared to control cells (blue), secondary only (light blue) and isotype control (orange).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody (ab215434)Immunohistochemical analysis of MCF-7 cells labeling p27 KIP 1 with ab215434 at 1/500 dilution in PBS for 2 hours at 37°C, followed by Goat anti-rabbit IgG (H+L), Cy3 conjugated secondary antibody. The nuclei were stained with DAPI.
