Anti-PRPF8/Prp8 antibody [2834C1a] (ab51366)
![Anti-PRPF8/Prp8 antibody [2834C1a] (ab51366)](https://www.abcam.com/ps/products/51/ab51366/Images/ab51366wb.jpg "Anti-PRPF8/Prp8 antibody [2834C1a] (ab51366)")
Key features and details
- Mouse monoclonal [2834C1a] to PRPF8/Prp8
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-PRPF8/Prp8 antibody [2834C1a]
See all PRPF8/Prp8 primary antibodies -
Description
Mouse monoclonal [2834C1a] to PRPF8/Prp8 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB MouseHuman
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Immunogen
Recombinant fragment corresponding to Human PRPF8/Prp8.
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Positive control
- NIH3T3 whole cell lysate, HeLa whole cell lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal cervix.
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General notes
Previously labelled as PRPF8.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Filtered through a 0.22 µm membrane. -
Clonality
Monoclonal -
Clone number
2834C1a -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-PRPF8/Prp8 antibody [2834C1a] (ab51366)Anti-PRPF8/Prp8 antibody [2834C1a] (ab51366) at 1/100 dilution + HeLa whole cell lysate at 25 µg
Secondary
Anti mouse IgG antibody at 1/2500 dilution
Predicted band size: 273 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [2834C1a] (ab51366)](https://www.abcam.com/ps/products/51/ab51366/Images/ab51366-195979-ab51366gr198315edited.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [2834C1a] (ab51366)IHC image of PRPF8/Prp8 staining in Human Normal Cervix formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51366, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Western blot - Anti-PRPF8/Prp8 antibody [2834C1a] (ab51366)](https://www.abcam.com/ps/products/51/ab51366/Images/ab51366wb2.jpg)
Western blot - Anti-PRPF8/Prp8 antibody [2834C1a] (ab51366)Anti-PRPF8/Prp8 antibody [2834C1a] (ab51366) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg
Secondary
Anti mouse IgG antibody at 1/2500 dilution
Predicted band size: 273 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
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![Flow Cytometry - Anti-PRPF8/Prp8 antibody [2834C1a] (ab51366)](https://www.abcam.com/ps/products/51/ab51366/Images/PRPF8-Primary-antibodies-ab51366-1.jpg)
Flow Cytometry - Anti-PRPF8/Prp8 antibody [2834C1a] (ab51366)Overlay histogram showing HeLa cells stained with ab51366 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51366, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
