Anti-ATP1A3 antibody [EPR14137] - BSA and Azide free (ab250625)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14137] to ATP1A3 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ATP1A3 antibody [EPR14137] - BSA and Azide free
See all ATP1A3 primary antibodies -
Description
Rabbit monoclonal [EPR14137] to ATP1A3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse and rat brain lysate. Human fetal brain lysate. Human hippocampus tissue lysate. IP: Human brain lysate.
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General notes
ab250625 is the carrier-free version of ab182572.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14137 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ATP1A3 antibody [EPR14137] - N-terminal (ab182572) at 1/5000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Human fetal brain lysate
Lane 4 : Human hippocampus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 111 kDaThis data was developed using ab182572, the same antibody clone in a different buffer formulation.
The 150 KDa band is caused by glycosylation which is consistent with what has been described in PMID: 22797008.
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This data was developed using ab182572, the same antibody clone in a different buffer formulation.
ATP1A3 was immunoprecipitated from 0.35 mg human brain lysate 10 ug with ab182572 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab182572 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.Lane 1: human brain lysate 10 ug
Lane 2: ab182572 IP in human brain lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab182572 in human brain lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds
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