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Neuroscience Neurotransmission Receptors / Channels Potassium Channels

Anti-ATP1A3 antibody [EPR14137] - BSA and Azide free (ab250625)

Anti-ATP1A3 antibody [EPR14137] - BSA and Azide free (ab250625)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14137] to ATP1A3 - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-ATP1A3 antibody [EPR14137] - BSA and Azide free
    See all ATP1A3 primary antibodies
  • Description

    Rabbit monoclonal [EPR14137] to ATP1A3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat brain lysate. Human fetal brain lysate. Human hippocampus tissue lysate. IP: Human brain lysate.
  • General notes

    ab250625 is the carrier-free version of ab182572.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14137
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Potassium Channels
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Sodium Channels
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases
    • Neuroscience
    • Processes

Images

  • Western blot - Anti-ATP1A3 antibody [EPR14137] - BSA and Azide free (ab250625)
    Western blot - Anti-ATP1A3 antibody [EPR14137] - BSA and Azide free (ab250625)
    All lanes : Anti-ATP1A3 antibody [EPR14137] - N-terminal (ab182572) at 1/5000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Human fetal brain lysate
    Lane 4 : Human hippocampus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 111 kDa



    This data was developed using ab182572, the same antibody clone in a different buffer formulation.

    The 150 KDa band is caused by glycosylation which is consistent with what has been described in PMID: 22797008.

  • Immunoprecipitation - Anti-ATP1A3 antibody [EPR14137] - BSA and Azide free (ab250625)
    Immunoprecipitation - Anti-ATP1A3 antibody [EPR14137] - BSA and Azide free (ab250625)

    This data was developed using ab182572, the same antibody clone in a different buffer formulation.

    ATP1A3 was immunoprecipitated from 0.35 mg human brain lysate 10 ug with ab182572 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab182572 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: human brain lysate 10 ug

    Lane 2: ab182572 IP in human brain lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab182572 in human brain lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds

  • Anti-ATP1A3 antibody [EPR14137] - BSA and Azide free (ab250625)
    Anti-ATP1A3 antibody [EPR14137] - BSA and Azide free (ab250625)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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